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目的:建立弥漫性腹膜炎动物模型,并观察不同程度弥漫性腹膜炎对肠黏膜通透性的影响。方法:采用升结肠支撑管腹膜炎模型(CASP)诱发弥漫性腹膜炎。将小鼠随机分为对照组、假手术组、和支撑管1.3mm组和1.7mm组。观察术后72h各组的生存率。并于术后18h比较肠黏膜通透性的变化。结果:升结肠支撑管内径越大,造成弥漫性腹膜炎小鼠死亡率越高。1.3mm组和1.7mm组术后72h累积生存率分别为75%和44%。1.7mm组肠道通透性高于1.3mm组。结论:CASP模型较好地模拟了急性弥漫性腹膜炎。当支撑管内径为1.7mm时,可模拟重度急性弥漫性腹膜炎,而当支撑管内径为1.3mm时则可模拟轻度急性弥漫性腹膜炎。
Objective: To establish an animal model of diffuse peritonitis and observe the effects of diffuse peritonitis on intestinal mucosal permeability. Methods: Diffuse peritonitis was induced by ascending colon proppant peritonitis model (CASP). The mice were randomly divided into control group, sham operation group, and support tube 1.3mm group and 1.7mm group. The survival rate of each group was observed 72h after operation. The changes of intestinal mucosal permeability were compared 18h after operation. Results: The larger the inner diameter of ascending colon support tube, the higher the mortality of mice with diffuse peritonitis. The cumulative survival rates at 72 hours after surgery in the 1.3 mm and 1.7 mm groups were 75% and 44%, respectively. 1.7mm group of intestinal permeability than the 1.3mm group. Conclusion: The CASP model better simulates acute diffuse peritonitis. Severe acute diffuse peritonitis can be simulated when the inner diameter of the support tube is 1.7 mm, and can simulate mild acute diffuse peritonitis when the inner diameter of the support tube is 1.3 mm.