Study on the Content of Forsythin,Total Flavonoids of Forsythia suspensa and Their Antioxidant Activ

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[Objective] To determine the content of forsythin and total flavonoids in different parts of Forsythia suspense,study their antioxidant activity,and analyze the relations of their content with antioxidant activity. [Method] Ultrasonic-assisted technology was applied to extract forsythin and total flavonoids from F.suspense. The content of forsythin and total flavonoids in F. suspense was respectively determined by means of HPLC and colorimetry. The antioxidant activity of theextracts from different parts of F. suspense towards free radical was evaluated by 1,1-diphenyl-2-icrylhydrazyl ( DPPH·) . [Result]The rank of different parts ofForsythia suspense in descending order was new leaves > old leaves > flowers > fruits according to the content of forsythin,while that was flowers > new leaves> old leaves > fruits according to the content of total flavonoids. Different parts of Forsythia suspensa had higher antioxidant activity,among which the antioxidantactivity of flower was the highest,followed by fruit,new leaf,and old leaf. [Conclusion]Extracts from F. suspensa flower,fruit and leaf all have higher antioxidant activity. Of these,total flavonoids are the primary active ingredients in flowers and leaves’ extract of F. suspense,and fruits’ extract is total flavonoids and forsythiaside. [Objective] To determine the content of forsythin and total flavonoids in different parts of Forsythia suspense, study their antioxidant activity, and analyze the relations of their content with antioxidant activity. [Method] Ultrasonic-assisted technology was applied to extract forsythin and total flavonoids from F.suspense. The content of forsythin and total flavonoids in F. suspense was determined determined by means of HPLC and colorimetry. The antioxidant activity of thetracts from different parts of F. suspense towards free radical was evaluated by 1,1-diphenyl- 2-icrylhydrazyl (DPPH ·). [Result] The rank of different parts of Forsythia suspense in descending order was new leaves> old leaves> flowers> fruits according to the content of forsythin, while that was flowers> new leaves> old leaves> fruits according to the content of total flavonoids. Different parts of Forsythia suspensa had higher antioxidant activity, among which the antioxidantactivity of flower was the highest , followed by fruit, new leaf, and old leaf. [Conclusion] Extracts from F. suspensa flower, fruit and leaf all have higher antioxidant activity. Of these, total flavonoids are the primary active ingredients in flowers and leaves’ extract of F. suspense, and fruits’ extract is total flavonoids and forsythiaside.
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