论文部分内容阅读
目的研究五味子乙素(Sch B)对阿尔茨海默病(AD)小鼠学习记忆能力及神经细胞凋亡的影响。方法 50只Bal B/C小鼠随机分为空白组、模型组、阳性药对照组、Sch B低浓度组和Sch B高浓度组。应用Morris水迷宫实验检测AD小鼠的学习记忆功能,HE染色观察AD小鼠脑组织病理形态学变化,流式细胞术和TUNEL染色观察小鼠脑组织神经细胞凋亡,Western印迹法及免疫组织化学法检测AD小鼠脑组织相关凋亡蛋白P53、Bax、Cyto C、C-caspase-9、C-caspase-3的表达。结果与模型组比较,应用Sch B小鼠水迷宫实验路径和潜伏期较短;HE染色显示,与模型组比较,应用Sch B干预后的AD小鼠海马神经细胞排列较为规整,细胞水肿明显减轻;流式细胞术和TUNEL染色结果显示,凋亡细胞比例明显减少;Western印迹法与免疫组织化学实验显示,Sch B干预后AD小鼠脑组织内P53、Bax、Cyto C、C-caspase-9、C-caspase-3蛋白的表达明显降低(P<0.01)。结论 Sch B能明显改善AD小鼠学习记忆能力,其机制可能与抑制神经细胞凋亡有关。
Objective To investigate the effects of Sch B on the learning and memory abilities and neuronal apoptosis in Alzheimer’s disease (AD) mice. Methods Fifty Bal B / C mice were randomly divided into blank group, model group, positive control group, Sch B low concentration group and Sch B high concentration group. Morris water maze test was used to detect the learning and memory function of AD mice. HE staining was used to observe the histopathological changes in brain tissue of AD mice. The apoptosis of brain tissue was observed by flow cytometry and TUNEL staining. Western blot and immunohistochemistry The expression of P53, Bax, Cyto C, C-caspase-9 and C-caspase-3 in brain tissues of AD mice was detected by chemical method. Results Compared with the model group, the water maze test route and incubation period of Sch B mice were short. The HE staining showed that compared with the model group, the hippocampal nerve cells arranged in Schizophrenia mice treated with Sch B were more regular and the edema of cells was significantly reduced. Flow cytometry and TUNEL staining showed that the percentage of apoptotic cells was significantly reduced. Western blotting and immunohistochemistry showed that the expression of P53, Bax, Cyto C, C-caspase-9, The expression of C-caspase-3 protein was significantly decreased (P <0.01). Conclusion Sch B can significantly improve the learning and memory abilities of AD mice, which may be related to the inhibition of neuronal apoptosis.