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利用DNA分子自组装技术可以构建从一维到三维不同形状的纳米结构,并且这些结构在微纳米电子学、纳米生物学等众多领域有许多潜在的用途.本文利用DNA分子瓦(tile)自组装技术,采用双交叉(DX)DNA分子瓦成功组装了一维DNA纳米管结构,聚丙烯酰胺凝胶电泳(native-PAGE)、透射电子显微镜(TEM)、荧光显微镜和原子力显微镜(AFM)对制得的DNA纳米管结构进行了表征,结果表明,组装成功的DNA纳米管直径在7～20nm之间,长度最长可以达到50μm以上.为了结构更加稳定,对分子瓦中每条DNA单链的5′末端进行磷酸化处理,自组装完成后利用T4DNA连接酶连接磷酸化修饰的DNA纳米管的缺口.AFM结果显示,使用T4DNA连接酶处理后的DNA纳米管更能保持完好的管状结构,表明连接处理后的DNA纳米管更加坚固,促进了DNA纳米管应用于微纳米领域的研究.
The use of DNA molecule self-assembly technology can be constructed from one-dimensional to three-dimensional nano-structures of different shapes, and these structures have many potential applications in many fields such as micro-nano electronics, nanobiology, etc. In this paper, Technology, one-dimensional DNA nanotube structure, native-PAGE, transmission electron microscopy (TEM), fluorescence microscopy and atomic force microscopy (AFM) were successfully assembled using double cross (DX) The results showed that the successful assembly of DNA nanotubes in the diameter of 7 ~ 20nm, the longest length can reach more than 50μm.In order to make the structure more stable, the molecular weight of each single-strand DNA tile 5 ’end of phosphorylation, after the completion of the assembly using T4 DNA ligase phosphorylation of modified DNA nanotubes gap.AFM results show that using T4 DNA ligase DNA nanotubes better able to maintain intact tubular structure, indicating that DNA nanotubes after attachment treatment are more robust, which has promoted the application of DNA nanotubes in the field of micro-nano.