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目的:对高效液相色谱法(High Performance Liquid Chrotomogrphy,HPLC)应用于测定中药成分溶度参数进行分析与探究。方法:利用指纹图谱理论以及Hildebrand-Scatchard溶解度理论创立HPLC测定中药成分的溶度参数,构建数学公式或模型,运用已知溶度参数的咖啡因验证理论对大黄类成分进行分析、测定。设置HPLC的条件为:Alltech Apollo C18柱子(高为25cm,直径为0.46cm,孔径为5μm)。其流动相为乙腈-水进行梯度洗脱,咖啡因的检测长度为430nm,柱子温度为25℃。结果:按照上述方法构建了指纹图谱几何平均保留时间与总量表观溶度参数关联的函数表达式或数学模型,然后根据这一数学表达式计算出所选取的中药成分的溶度参数值大小,其值为37.55J1/2/cm3/2。结论:高效液相色谱(HPLC)指纹图谱构建数学模型方法,能够同时对中药多个组分进行分析、测定,从而能够为单味中药药剂的制造工艺提供一定的理论基础与测定技术方面的支持。
Objective: To analyze and explore the determination of solubility parameters of traditional Chinese medicine by using High Performance Liquid Chromogrphy (HPLC). Methods: The fingerprint parameters theory and Hildebrand-Scatchard solubility theory were used to establish the HPLC method to determine the solubility parameters of traditional Chinese medicines. The mathematical formulas or models were established. The caffeine verification theory with known solubility parameters was used to analyze and determine the rhubarb components. The conditions for setting up the HPLC were: Alltech Apollo C18 column (height 25 cm, diameter 0.46 cm, pore size 5 μm). The mobile phase was acetonitrile-water gradient elution, the caffeine detection length was 430 nm, and the column temperature was 25°C. Results: According to the above method, the functional expression or mathematical model associated with the geometric mean retention time of the fingerprint and the total apparent solubility parameter was constructed. Then the solubility parameter value of the selected Chinese medicine component was calculated based on this mathematical expression. Its value is 37.55 J1/2/cm3/2. Conclusion: High-performance liquid chromatographic (HPLC) fingerprinting can be used to construct a mathematical model method, which can simultaneously analyze and determine multiple components of traditional Chinese medicines, thus providing a certain theoretical basis and technical support for the manufacturing process of single-taste traditional Chinese medicines. .