论文部分内容阅读
目的 :了解灭活SARS病毒肌肉注射途径对系统免疫的影响。方法 :将 5周龄雌性BALB/c小鼠随机分为两组 ,每组 1 0只 ,免疫组小鼠每只注射 5 0 μg的灭活病毒悬液 ,对照组注射等体积PBS。每组分别在免疫的 8d和1 4d处死 5只小鼠 ,分离脾细胞和外周血淋巴细胞 ,流式细胞仪观察其淋巴细胞表型。结果 :第 8天脾脏的淋巴细胞表型变化较为明显 ,CD+ 4 T细胞的构成比增加 (P =0 .0 0 1 ) ,CD+ 4 /CD+ 4 比值明显增多 (P =0 .0 0 7)。外周血淋巴细胞表型在第 1 4天变化明显 ,与对照组有极显著差异 ,CD+ 4 ,CD+ 8,CD+ 3 T细胞构成比和T/B比值显著降低 (P =0 .0 0 5 ,0 .0 0 0 ,0 .0 0 6和 0 .0 0 1 ,) ,同时CD4 5R/B2 2 0 + 细胞 (B细胞 )构成比显著增加 (P =0 .0 0 0 )。结论 :肌肉途径免疫灭活SARS病毒早期即可诱导机体系统免疫细胞表型的变化 ,推测早期可以诱导体液免疫应答
Objective: To understand the effect of inactivated SARS virus intramuscular injection on systemic immunity. Methods: Five-week-old female BALB / c mice were randomly divided into two groups of 10 mice in each group. The mice in the immunized group were injected with 50 μg of inactivated virus suspension and the control group with equal volume of PBS. Five mice were sacrificed on day 8 and day 14 respectively after immunization. Splenocytes and peripheral blood lymphocytes were isolated and their lymphocyte phenotypes were observed by flow cytometry. Results: On the 8th day, the spleen lymphocyte phenotype changed obviously, the ratio of CD + 4 T cells was increased (P = 0.010) and the ratio of CD + 4 / CD + 4 was significantly increased . The phenotypes of peripheral blood lymphocytes changed significantly on the 14th day, and were significantly different from that of the control group (P = 0.0500, P <0.05). The ratios of CD + 4, CD + 8, CD + 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Conclusion: Muscle pathway immunization inactivated SARS virus can induce the change of immune system phenotype in the early stage of the body, suggesting that early humoral immune response can be induced