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2005—2006年对云南省主要蔬菜种植区昆明、红河和楚雄等3个地区进行调查,获得9份疑似番茄斑萎病毒属(Tospovirus)病毒的样本。通过该属病毒引物(Tospovirus Group Primer)进行RT-PCR扩增,其中1份来自红河州建水县番茄上的样品获得约500bp核酸片段,通过NCBIBLAST网络数据库比对,确定为Tospovirus属的病毒;应用DNAMAN软件与已经公布的Tospovirus属病毒LRNA序列比对分析,从得到的相似性树状图可以看出,该样品与血清组Ⅳ的Tospovirus属病毒聚为一簇。
From 2005 to 2006, 3 samples of Kunming, Honghe and Chuxiong, the major vegetable growing areas in Yunnan Province, were surveyed to obtain 9 samples of suspected tomato (Toxoplasma gondii) virus. RT-PCR amplification was carried out by the Tospovirus Group Primer. One copy of a 500 bp DNA fragment from tomato in Jianshui County of Honghe Prefecture was identified as a virus belonging to the genus Tospovirus by NCBIBLAST internet database. Using DNAMAN software and published sequence analysis of Tospovirus genus LRNA, we can see from the similar dendrogram that this sample clustered with the Tospovirus of serogroup IV.