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目的探讨肺炎衣原体(CPn)感染与动脉粥样硬化(AS)的相关性。方法小鼠随机分为正常组、感染组、高脂组、高脂感染组。喂饲高胆固醇饲料1周后,经一侧鼻腔滴入含CPn的培养液,每周1次,共3次。第1次接种后第18周末处死全部动物,取标本进行相关指标检测。结果感染组、高脂组、高脂感染组的肿瘤坏死因子-α(TNF-α)分别为(7.196±0.436),(4.236±0.408),(8.881±0.529)pg/ml;高脂感染组白细胞介素-10(IL-10)、可溶性血管细胞粘附分子-1(sVCAM-1)分别为(541.30±99.97),(8.176±0.489)pg/ml;感染组、高脂感染组白细胞介素-6(IL-6)分别为(89.70±9.35),(123.30±8.97)pg/ml;可溶性细胞间粘附分子-1(sICAM-1)分别为(4.545±1.232),(6.131±1.083)pg/ml;可溶性E-选择素(sE-selectin)分别为(297.10±93.72),(405.50±45.59)pg/ml;与正常组比较,P<0.01。感染组和高脂组TNF-α分别为(7.196±0.436),(4.236±0.408)pg/ml;IL-6分别为(89.70±9.35),(77.20±6.97)pg/ml;IL-10分别为(249.20±124.84),(191.80±72.37)pg/ml;sICAM-1分别为(4.545±1.232),(2.966±1.511)pg/ml;sVCAM-1分别为(6.672±0.421),(6.714±0.944)pg/ml;sE-selectin分别为(297.10±93.72),(234.30±45.68)pg/ml;与高脂感染组比较,均P<0.01。3个观察组内皮细胞损伤程度与正常组比较,均P<0.01;感染组内皮细胞损伤程度与高脂感染组比较,P<0.05。高脂组和高脂感染组斑块面积指数(PAI)分别为(13.90±6.58),(15.53±7.20),与正常组比较,均P<0.01。对乙酰胆碱舒张血管作用的反应性,高脂组(46.13±5.76)与正常组(66.85±6.37)比较,P<0.01;高脂感染组与正常组、感染组和高脂组比较,均P<0.01。结论CPn感染有增强高脂饮食致动脉粥样硬化的作用。
Objective To investigate the relationship between Chlamydia pneumoniae (CPn) infection and atherosclerosis (AS). Methods Mice were randomly divided into normal group, infection group, high fat group and high fat group. One week after feeding high-cholesterol diet, nasal instillation of culture solution containing CPn once a week for 3 times. After the first vaccination, all animals were sacrificed at the end of the 18th week, and samples were taken for the detection of related indicators. Results The levels of tumor necrosis factor-α (TNF-α) were (7.196 ± 0.436), (4.236 ± 0.408) and (8.881 ± 0.529) pg / ml in the infected group, The levels of interleukin-10 (IL-10) and soluble vascular cell adhesion molecule-1 (sVCAM-1) were 541.30 ± 99.97 and 8.176 ± 0.489 pg / The levels of sICAM-1, sICAM-1 and sICAM-1 were (89.70 ± 9.35) and (123.30 ± 8.97) pg / ml, ) pg / ml, sE-selectin were (297.10 ± 93.72) and (405.50 ± 45.59) pg / ml respectively, P <0.01 compared with the normal group. The levels of TNF-α in the infected group and the hyperlipidemic group were (7.196 ± 0.436) and (4.236 ± 0.408) pg / ml respectively, while the levels of IL-6 were (89.70 ± 9.35 and 77.20 ± 6.97) pg / (249.20 ± 124.84) and (191.80 ± 72.37) pg / ml respectively; sICAM-1 was (4.545 ± 1.232) and (2.966 ± 1.511) pg / ml respectively; sVCAM-1 was (6.672 ± 0.421) and 0.944) pg / ml and sE-selectin were (297.10 ± 93.72) and (234.30 ± 45.68) pg / ml, respectively. Compared with the high fat-infected group, all the differences were statistically significant , Both P <0.01; The level of endothelial cell injury in infected group was significantly higher than that in high-fat group (P <0.05). The plaque area index (PAI) of high fat group and high fat group were (13.90 ± 6.58) and (15.53 ± 7.20) respectively, both P <0.01 compared with the normal group. The response to acetylcholine in vasodilatation was higher in high-fat group (46.13 ± 5.76) than in normal group (66.85 ± 6.37), P <0.01. Compared with normal group, infection group and high-fat group, 0.01. Conclusions CPn infection can enhance the atherosclerosis induced by high-fat diet.