Protection of Guinea Pigs against Leptospira interrogans Serovar Lai by LipL21 DNA Vaccine

来源 :中国免疫学杂志 | 被引量 : 0次 | 上传用户:cdelphiboy
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
In this study,the full lipL21 gene fragment encoding outer membrane protein LipL21 was cloned from L. Interrogans serovar Lai and inserted into eukaryotic expression vector pcDNA3.1(+).The guinea pigs were immunized with pcDNA3.1(+)-lipL21,pcDNA3.1(+) or PBS.Six weeks after the second immunization,the splenocytes were isolated to detect their proliferative ability by lymphocyte transformation experiments.In addition,microscopic agglutination test was used for quantitative detection of specific antibodies.The rest guinea pigs were challenged intraperitoneally with L.interogans sorevar Lai.Then,protective effect was evaluated on the basis of survival and histopathological lesions in the kidneys,lungs,and liver.The lipL21 gene Was successfully expressed in COS-7 cells through recombinant pcDNA3.1(+)-lipL21.The titer of specific antibodies substantially increased,and the stimulation index of splenocytes increased significantly.Hence,the pcDNA3.1(+)-lipL21 could protect the immunized guinea pigs from homotypic Leptospira infection.Furthermore,no obvious pathologic changes were observed in the pcDNA3.1(+)-lipL21 immunized guinea pigs.The results showed that the protective effect with pathogenic strains of Leptospira was shared by LipL21 mediated through a plasmid vector. Consequently,these results indicated that the lipL21 DNA vaccine Was a promising candidate for the prevention of leptospirosis.
其他文献
欧债危机对我国经济造成重大影响,一方面加大了人民币的升值压力,使中国对欧洲国家的出口大幅下降;另一方面也迫使中国转变经济发展方式以减少对出口的依赖。面对欧债危机,我
Epstein-Barr virus(EBV),a potential oncogenic herpesvirus,has been found to be associated with several malignancies.Its critical to elicit cellular immunity of
Summary: The aim of this study was to investigate the in vitro cytotoxieity of polyphosphoester polymer used as a novel injectable alveolar bone substitutes for
随着低碳的概念深入人心,低碳旅游也成为了当先旅游界的一个新坐标,以低碳为视角发展休闲农业旅游本身就是对传统乡村旅游的一种升华。本文通过对国内外休闲农业旅游发展历史
The China Advanced Research Reactor (CARR) is scheduled to be operated in the autumn of 2008. In this paper, we report preparations for installing the neutron r
Objective To explore the vulnerability to HIV infection and related risk behaviors of the out-of-school adolescents who migrated with their parents from rural
改革开放后民族经济政策的稳定是相对的、总体的稳定。这一时期党和国家对少数民族和民族地区的发展理念、客观现实及国家帮助方针、对口支援方针、因地制宜原则、自力更生原
Objective To explore the migration of transplanted neural stem cells co-labeled with superparamagnetic iron oxide (SPIO) and bromodeoxyuridine (Brdu) using
基于知识转移过程理论和技术联盟过程中所呈现的特征和相关影响因素,提出了技术联盟知识转移影响因素概念模型.通过实际采集的数据对技术联盟过程中影响知识转移效果的因素进
Objective To establish an experimental model of early stage avascular necrosis of the femoral head (ANFH) caused by corticosteroid in adult rabbits and to obse