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目的:建立氧化石墨(GO)纳米粒中10-羟基喜树碱(HCPT)含量测定的高效液相色谱方法。方法:色谱柱:Diamonsil~(TM)ODS-C_(18)(200 mm×4.6 mm,5μm),流动相:乙腈-水(25:75),流速:1.0 ml·min~(-1),检测波长:266 nm。结果:在本试验条件下制剂中辅料对HCPT含量的测定无干扰,HCPT的质量浓度在1～100μg·ml~(-1)内线性关系良好(r=0.999 9),平均回收率为99.54%。GO纳米粒中HCPT含量可达到(0.157±0.009)mg·mg~(-1)。结论:含量测定方法准确可靠、简单易行,可用于GO纳米粒中HCPT的含量测定。
Objective: To establish a high performance liquid chromatography (HPLC) method for the determination of 10-hydroxycamptothecin (HCPT) in graphite oxide (GO) nanoparticles. METHODS: Diamonsil TM ODS-C 18 (200 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and water (25:75) at a flow rate of 1.0 ml · min -1. Detection wavelength: 266 nm. Results: There was no interference with the determination of HCPT content in the preparations under the conditions of this test. The linearity of HCPT was in the range of 1 ~ 100μg · ml ~ (-1) (r = 0.999 9) with the average recovery of 99.54% . The content of HCPT in GO nanoparticles can reach (0.157 ± 0.009) mg · mg ~ (-1). Conclusion: The content determination method is accurate, reliable and simple. It can be used for the determination of HCPT in GO nanoparticles.