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目的腹主动脉缩窄法(AO)建立大鼠慢性心力衰竭模型,研究B型钠尿肽(BNP)在评价大鼠心功能中的作用。方法Wistar大鼠80只,随机分为手术组和假手术组,术后第4、8、12和16周时行血流动力学监测,留取血清和病理标本,比较血流动力学指标、病理和血清学指标。结果与假手术组相比,大鼠AO术后4周,BNP水平显著升高(P<0.05),并在观察期间进行性升高,心肌细胞直径(CMD)、心脏质量指数升高。术后8周-dp/dtmax降低。术后12周LVSP升高,+dp/dtmax降低。术后16周上述改变更明显。BNP水平与血流动力学和心肌肥厚指标相关性良好。结论腹主动脉缩窄可成功复制大鼠慢性心力衰竭模型,BNP对模型大鼠心功能受损高度敏感。
Objective To establish an animal model of chronic heart failure by abdominal aorta constriction (AO) and to study the role of B-type natriuretic peptide (BNP) in the evaluation of cardiac function in rats. Methods Eighty Wistar rats were randomly divided into operation group and sham operation group. After 4, 8, 12 and 16 weeks after operation, hemodynamic monitoring was performed. Serum and pathological specimens were collected. The hemodynamics, Serological markers. Results Compared with the sham-operation group, BNP level was significantly increased 4 weeks after AO in rats (P <0.05), and increased progressively during the observation period. Cardiac cell diameter (CMD) and heart mass index increased. After 8 weeks, the -dp / dtmax decreased. After 12 weeks LVSP increased, + dp / dtmax decreased. 16 weeks after the above change is more obvious. BNP levels correlate well with hemodynamics and cardiac hypertrophy. Conclusion Abdominal aortic constriction can be successfully replicated in chronic heart failure model rats. BNP is highly sensitive to impaired cardiac function in model rats.