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目的 探讨人白血病细胞系Ｕ９３７ 的白血病抑制因子（ＬＩＦ）受体α亚基和ｇｐ１３０ 亚基细胞内区与转录活性因子Ｓｔａｔ３ 的活性关系，旨在研究白血病细胞增殖和分化的机制。方法 用基因重组技术将２ 个基因在细胞内区截除（ｇｐ１９０ＥＸ、ｇｐ１３０ＥＸ），并分别在Ｕ９３７ 细胞中表达，因其可与野生型受体竟争性结合ＬＩＦ，故可用免疫印迹法分析受体细胞内区截除后Ｓｔａｔ３ 的表达水平及该信号分子酪氨酸磷酸化。结果 转染ｇｐ１９０ＥＸ 和ｇｐ１３０ＥＸ 的Ｕ９３７ 细胞Ｓｔａｔ３ 表达量增加；Ｓｔａｔ３ 酪氨酸磷酸化水平下降。结论 ＬＩＦ受体α亚基和ｇｐ１３０ 的细胞内区均参与了Ｓｔａｔ３ 的激活和Ｕ９３７ 细胞的分化。
Objective To investigate the relationship between the intracellular domain of leukemia inhibitory factor (LIF) receptor alpha subunit and gp130 subunit of human leukemia cell line U937 and the activity of transcriptional activator Stat3 in order to study the mechanism of leukemia cell proliferation and differentiation. Methods Two genes (gp190EX, gp130EX) were cut off in the intracellular region by gene recombination technique and expressed respectively in U937 cells. Because of their competitive binding to wild-type receptors, LIF could be analyzed by immunoblotting The expression of Stat3 and the signal tyrosine phosphorylation after the excision of the intracellular region. Results The expression of Stat3 in U937 cells transfected with gp190EX and gp130EX cells was increased, and the level of Stat3 tyrosine phosphorylation was decreased. Conclusion Both LIF receptor α subunit and the intracellular domain of gp130 are involved in the activation of Stat3 and the differentiation of U937 cells.