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以2年生北高丛蓝莓(Vaccinium corymbosum L.)3个优良品种“蓝丰”、“公爵”和“布里吉塔”的苗木为实验材料,利用人工气候箱控制生长温度,探讨不同温度处理对北高丛蓝莓叶片气体交换和叶绿素荧光参数的影响。结果表明:随着温度升高,3个品种叶片的净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)和水分利用效率(WUE)均先升高后降低,最大值随品种的不同而发生变化;3个品种的细胞间CO_2浓度(C_i)均一致性地呈现相反的变化趋势,30和35℃高温使Ci降低,而40℃高温又导致了Ci升高;相对电导率和叶绿素荧光参数F_v/F_m的测定结果表明,30和35℃高温对北高丛蓝莓叶片结构和功能的影响非常有限,40℃高温却导致其细胞膜结构的破坏;40℃高温对光系统Ⅱ反应中心及其电子传递链的结构造成了不可逆的严重损伤;不同北高丛蓝莓品种的抗高温能力存在很大差异,“布里吉塔”最强,“蓝丰”次之,“公爵”最弱。研究结果有助于深入理解温度对北高丛蓝莓产生影响的潜在机理,为蓝莓耐高温品种选育和引种工作提供理论依据。
Three seedlings of 2-year-old Vaccinium corymbosum L., “Lanfeng”, “Duke” and “Bridgita”, were used as experimental materials, and artificial climate chamber was used to control the growth temperature The effects of different temperature treatments on the gas exchange and chlorophyll fluorescence parameters of blueberry leaves in North Highland were investigated. The results showed that the net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r) and water use efficiency (WUE) of three varieties increased firstly and then decreased with the increase of temperature. The maximum The CO 2 concentration (C_i) in all three cultivars consistently showed the opposite trend. The high temperature at 30 and 35 ℃ decreased Ci, while the high temperature at 40 ℃ caused the increase of Ci. The relative conductance The results showed that the effects of 30 and 35 ℃ on the structure and function of blueberry blueberry leaves were very limited. The high temperature of 40 ℃ led to the destruction of the cell membrane structure. The effects of high temperature of 40 ℃ on the photosystem Ⅱ The structure of the reaction center and its electron transfer chain caused irreversible serious damage. There are great differences in the anti-high-temperature abilities of different varieties of North Highland blueberry varieties, followed by “Brigitta” and “Lanfeng” “Duke ” the weakest. The results will help to understand the underlying mechanism of the temperature on the impact of the North High Blueberry, blueberry for breeding and introduction of high temperature varieties provide a theoretical basis.