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目的:探讨原花青素(PC)对氨基脲(SEM)染毒小鼠精子质量损伤的修复作用。方法:50只清洁级成年雄性昆明种小鼠按体重随机平分成5组(10只/每组):溶剂对照组、SEM染毒组、s EM染毒加低、中、高剂量PC保护组。溶剂对照组小鼠灌胃去离子水;SEM染毒组小鼠按56.25 mg/kg?bw剂量灌胃SEM溶液;低、中、高剂量PC保护组小鼠分别按100、200、400 mg/kg?bw剂量灌胃PC溶液。除溶剂对照组灌胃等体积的去离子水外,其它每组均上午灌胃SEM、下午灌胃PC,每天1次,实验时间均为6周。实验结束后取血后处死动物,分离双侧睾丸、附睾测定相关指标。结果:SEM染毒组小鼠体重及睾丸、附睾脏器系数均低于溶剂对照组(P<0.05);经不同剂量PC保护后,PC高剂量保护组小鼠体重及附睾、睾丸脏器系数均分别高于SEM染毒组(P<0.05);与溶剂对照组比较,SEM染毒组小鼠精子畸形率增加,精子数目、活动度降低(P<0.05);经高剂量PC保护后小鼠精子数目、活动度均较SEM染毒组增加,畸形率减少(P<0.05),精子畸形以折体、弯颈、卷尾、不定型、双头、胖头为主;SEM染毒组SOD降低,MDA升高;与染毒组比较高剂量PC保护后SOD升高,MDA降低,差异有统计学意义(P<0.05)。结论:s EM对小鼠精子质量有损伤作用;400 mg/kg?bw剂量PC对SEM损伤小鼠精子质量有较好的修复作用,PC作用机制可能与其抗氧化作用有关。
Objective: To investigate the effect of proanthocyanidins (PC) on the repair of sperm quality damage induced by semicarbazide (SEM) in mice. Methods: Fifty clean adult male Kunming mice were randomly divided into 5 groups (10 mice / group) according to body weight: solvent control group, SEM exposure group, s EM exposure plus low, middle and high dose PC protection group . The mice in the solvent control group were given gavage with deionized water. The mice in the SEM group were dosed with the SEM solution at a dose of 56.25 mg / kg bw. The mice in the low, medium and high dose PC protection groups were treated with 100, 200, 400 mg / kg? bw dose of intragastric administration of PC solution. Except for the solvent control group, the same volume of deionized water was intragastrically administered, and each group was given SEM in the morning and PC in the afternoon, once a day for 6 weeks. After the experiment, blood was sacrificed and animals were sacrificed. Bilateral testes and epididymal indexes were measured. Results: The body weight, testis and epididymis parameters of mice in SEM group were significantly lower than those in the solvent control group (P <0.05). After protection with different doses of PC, the body weight, epididymis and testis organ coefficient (P <0.05). Compared with the solvent control group, the sperm deformity rate and the sperm count and the activity of the mice in the SEM group were significantly decreased (P <0.05). After high-dose PC protection Sperm motility and sperm motility in SEM group were higher than those in SEM group (P <0.05). Sperm malformations were mainly in the form of zigzag, curved neck, SOD decreased and MDA increased. Compared with the exposed group, the SOD increased and the MDA decreased after high-dose PC protection, the difference was statistically significant (P <0.05). CONCLUSION: s EM can damage sperm quality in mice. PC at a dose of 400 mg / kg? Bw can repair sperm in SEM mice, and the mechanism of PC may be related to its antioxidative effect.