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设计并合成了一种以罗丹明B为基础的Hg~(2+)荧光探针:3’,6’-双(二乙基氨基)-2-(-2-1,3,3-三甲基二氢吲哚-2-甲基)氨基)螺[异二氢吲哚-1,9’-呫吨]-3-酮(L1)。在乙醇和水50:50(V:V)的体系中,探针L1对Hg~(2+)表现出高的选择性,当加入10倍当量的Hg~(2+)时,荧光强度增加大约60倍。另外当加入一定量的Hg~(2+)后,可以裸眼观察到溶液颜色由无色变为粉红色。Hg~(2+)浓度在0.80~10μmol/L的范围内具有良好的线性关系,检出限为0.37μmol/L。溶液中除Cu~(2+)之外的其它金属离子对Hg~(2+)的测量几乎没有干扰,探针对Hg~(2+)的检测具有良好的选择性。光谱滴定表明,Hg~(2+)与探针L1形成1:1配合物,同时对该探针进行了实际应用,可用来检测自来水等样品中Hg~(2+)的含量。
We designed and synthesized a Hg 2+ fluorescent probe based on rhodamine B: 3 ’, 6’-bis (diethylamino) -2 - (- Methylindolin-2-methyl) amino) spiro [isoindoline-1,9’-xanthen] -3-one (L1). In the system of ethanol and water 50:50 (V: V), probe L1 showed high selectivity to Hg 2+, and fluorescence intensity increased when 10-fold equivalent of Hg 2+ was added About 60 times. In addition, when a certain amount of Hg 2+ is added, the color of the solution can be changed from colorless to pink by the naked eye. The concentration of Hg 2+ in the range of 0.80 ~ 10μmol / L has a good linear relationship with a detection limit of 0.37μmol / L. The other metal ions except Cu2 + have almost no interference with the measurement of Hg2 +, and the probe has good selectivity for the detection of Hg2 +. The results of spectral titration showed that Hg 2+ formed a 1: 1 complex with probe L1. At the same time, the probe was applied to detect the content of Hg 2+ in tap water and other samples.