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目的:观察人参皂苷Rgl联合美满霉素对脑缺血大鼠学习与记忆的影响和海马NMDA受体亚单位(NR2A/B)表达的变化。方法:本研究将48只成年雄性SD大鼠分为正常组、假手术组、慢性脑缺血组、人参皂苷Rgl+美满霉素处理组,每组12只。利用双侧颈总动脉结扎方法制备慢性脑缺血再灌注大鼠模型,造模后药物处理21 d,采用Morris水迷宫和及穿梭箱实验测试其学习与记忆成绩,再采用Western Blot方法分析海马的NR2A,NR2B的表达。结果:Morris水迷宫测试结果显示模型组大鼠寻找平台的潜伏期较假手术组明显延长,药物处理组大鼠寻找平台的潜伏期较模型组明显缩短;穿梭箱测试结果显示:与假手术组相比,模型组大鼠电击时间明显延长;与模型组相比,治疗组大鼠电击时间明显缩短;Western Blot结果显示:模型组NR2A表达水平较假手术组明显降低,而NR2B明显升高;药物处理组大鼠海马内NR2A表达水平较模型组明显上调,而NR2B明显下调。结论:人参皂苷Rgl联合美满霉素明显改善脑缺血大鼠的学习记忆能力,其作用机制可能与调节NMDA受体表达有关。
Objective: To observe the effects of ginsenoside Rgl and minocycline on learning and memory and the expression of NMDA receptor subunit (NR2A / B) in hippocampus of rats with cerebral ischemia. Methods: In this study, 48 adult male SD rats were divided into normal group, sham operation group, chronic cerebral ischemia group and ginsenoside Rgl + minocycline group, with 12 rats in each group. The rat models of chronic cerebral ischemia / reperfusion were established by bilateral common carotid artery ligation. After modeling for 21 days, Morris water maze and shuttle box were used to test the learning and memory performance. Western Blot was used to analyze the hippocampus Of NR2A, NR2B expression. Results: The results of Morris water maze test showed that the latent period of platform searching for rats in model group was significantly longer than that in sham operation group. The incubation period of rats in drug treatment group was significantly shorter than that in model group. Shuttle box test showed that compared with sham operation group , The shock time of rats in model group was significantly longer than that in model group. Compared with model group, the shock time of rats in treatment group was significantly shortened. Western Blot showed that the expression of NR2A in model group was significantly lower than that in sham group and NR2B, The expression of NR2A in hippocampus of rats in the model group was significantly higher than that of the model group, while NR2B was significantly down-regulated. CONCLUSION: Ginsenoside Rgl combined with minocycline can significantly improve the learning and memory abilities of rats with cerebral ischemia. Its mechanism may be related to the regulation of NMDA receptor expression.