体外培养的心肌细胞可保持其结构及功能上的某些特点,具有自发性节律搏动,已广泛用于生物学及医学研究,近年国外发展较快,为从细胞及分子水平研究心肌的生理、病理、药理以及某些基本理论问题,开辟了一个新的领域,早在1910年Burrows氏首创鸡胚心肌组织块培养,观察到搏动。两年后Carrel维持鸡胚心肌组织块连续搏动达104天,并做传代培养。他退休后由Ebeling继续培养达34 In vitro cultured cardiomyocytes can maintain some characteristics of their structure and function, with spontaneous rhythm of beating, has been widely used in biology and medical research, rapid development in foreign countries in recent years, from the cellular and molecular levels of cardiac physiology, Pathology, pharmacology and some basic theoretical issues opened up a new field, as early as 1910, Burrows’s first chick embryo myocardial tissue mass culture, the beating was observed. Two years later, Carrel maintained a continuous pulsation of chick embryo cardiomyocytes for 104 days and was subcultured. After he retired, Ebeling continued to train up to 34