许多病毒——寄主组合,在生长点大约100～1000um的音口位,并不含病毒或只含少量,可切下供组织培养,以获得无病毒营养系。柑桔茎顶嫁接技术的发展是针对柑桔茎顶组织培养不易发根的缺点,采取的技术措施。砧木幼苗原先培养在试管内,在胚茎切断面处,切成倒T形伤口,然后把优良品种柑桔顶梢0.14mm Many virus - host combinations, at the growing point of about 100 ~ 1000um tone sound mouth, and does not contain viruses or only a small amount, can be cut for tissue culture, in order to obtain virus-free nutrition. The development of citrus stems grafting technology is aimed at the shortcomings of citrus stem tissue culture is not easy to root, the technical measures taken. Rootstock seedlings were originally cultured in vitro, the cut surface of the embryo stem, cut into inverted T-shaped wound, and then the top varieties of fine varieties of citrus 0.14mm