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背景与目的:NAG7基因是我室克隆的鼻咽癌相关的肿瘤抑制候选基因,其功能与作用机制目前尚不清楚。研究鼻咽癌相关的潜在抑瘤基因NAG7对鼻咽癌细胞系(HNE1)细胞周期和细胞凋亡的影响,并探讨其作用机制。方法:采用脂质体转染技术将NAG7基因导入HNE1细胞,建立稳定表达NAG7的细胞株,Northernblot分析转染细胞NAG7基因的表达,并采用流式细胞技术检测细胞周期、细胞周期素及细胞凋亡的改变,并用westernblo验证。结果:NAG7基因重表达的HNE1细胞与空载体转染的HNE1和HNE1细胞相比:G0/G1期细胞数增加(P0.05),S期细胞数减少;细胞凋亡数目增加(P<0.05);细胞周期素A、D1、E表达明显降低(P<0.05),细胞周期素B1表达降低,Westernblot检测亦证实cyclin-D1和cyclin-E表达明显下调。结论:NAG7的重表达导致细胞周期素表达下调,从而延缓细胞经G1期进入S周期及诱导细胞凋亡增加进而抑制NPC细胞的过度增殖。
BACKGROUND & AIM: The NAG7 gene is a candidate tumor suppressor gene associated with nasopharyngeal carcinoma cloned in our laboratory. Its function and mechanism of action are not yet clear. To investigate the effect of NAG7, a potential tumor suppressor gene associated with nasopharyngeal carcinoma, on the cell cycle and apoptosis of nasopharyngeal carcinoma cell line (HNE1) and to explore its mechanism. Methods: The NAG7 gene was transfected into HNE1 cells by lipofection technique to establish a cell line stably expressing NAG7. The expression of NAG7 gene was detected by Northern blot analysis. The cell cycle, cyclin and cell apoptosis were detected by flow cytometry Change of death, and verified with westernblo. Results: Compared with HNE1 and HNE1 transfected with empty vector, the number of cells in G0 / G1 phase increased (P <0.05), the number of cells in S phase decreased and the number of apoptotic cells increased (P <0.05 ). The expressions of cyclin D1 and cyclin-E were significantly downregulated (P <0.05), and the expression of cyclin B1 was decreased. CONCLUSION: NAG7 overexpression leads to the down-regulation of cyclin expression, which delays cell entry into S-cycle through G1 phase and induces an increase in apoptosis, thus inhibiting the excessive proliferation of NPC cells.