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目的观察高原低氧对大鼠肺组织超微结构影响及其低氧诱导因子1α(HIF-1α)表达变化。方法将SD大鼠分别进行高原低氧干预1、2、3和30 d,并设置对照组;4个高原低氧组由海拔5 m的西安地区途中耗时1d带到海拔2 700 m青海格尔木地区、途中耗时2 d带到海拔5 000 m唐古拉地区,途中耗时3d带到海拔4 500 m的西藏那曲地区,并饲养30 d。结果光镜及电镜观察显示,急性高原低氧2 d组肺组织出现明显高原肺水肿,高原低氧30 d组HIF-1αmRNA条带积分吸光度值(0.874±0.075)明显高于对照组(0.225±0.026)(P<0.01),高原肺水肿现象则明显减轻。结论低氧习服后肺组织低氧诱导因子1αmRNA表达升高,有利于减轻高原肺水肿。
Objective To observe the effects of plateau hypoxia on the ultrastructure of lung tissue and the expression of hypoxia inducible factor 1α (HIF-1α) in rats. Methods SD rats were exposed to high altitude hypoxia for 1, 2, 3 and 30 days, respectively, and the control group was set up. The 4 plateau hypoxia groups were taken from Xi’an, 5 m above sea level, Area and took 2 days on the way to the Tanggula region at 5 000 m above sea level. This took about 3 days to reach Nagqu, Tibet, at an altitude of 4 500 m and was kept for 30 days. Results The results of light microscope and electron microscope showed that pulmonary alveolus of lung in 2 days acute hypoxia group was significantly higher than that in control group (0.874 ± 0.075) 0.026) (P <0.01), the phenomenon of high altitude pulmonary edema was significantly reduced. Conclusion The hypoxia-inducible factor 1α mRNA expression in lung tissue increased after hypoxia acclimatization, which is beneficial to reduce altitude pulmonary edema.