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目的观察姜黄素对猪链球菌诱导的小鼠脾细胞促炎性细胞因子的影响,探讨姜黄素对猪链球菌引起的急性中毒性休克的干预作用。方法采用乳酸脱氢酶释放法检测猪链球菌和姜黄素的细胞毒作用,酶联免疫吸附试验和流式细胞术检测相关炎症因子。结果所选剂量猪链球菌和姜黄素均未发现明显细胞毒作用。猪链球菌诱导脾细胞产生了促炎性细胞因子IL-6、TNF-α、IL-12和较高剂量的IFN-γ以及少量的IL-1β,同时也产生了抗炎因子IL-10。姜黄素对促炎因子都有抑制作用同时降低了促炎因子与抗炎因子的比例。结论姜黄素能够降低猪链球菌诱导小鼠脾细胞产生的促炎因子而发挥抗炎作用。
Objective To observe the effect of curcumin on the proinflammatory cytokines induced by Streptococcus suis in mice and explore the intervention of curcumin on acute toxic shock caused by Streptococcus suis. Methods The cytotoxicity of streptococcus suis and curcumin was detected by lactate dehydrogenase release assay. The related inflammatory cytokines were detected by enzyme - linked immunosorbent assay and flow cytometry. Results The dose of selected Streptococcus suis and curcumin were not found significant cytotoxicity. Streptococcus suis induced splenocytes to produce proinflammatory cytokines IL-6, TNF-α, IL-12 and higher doses of IFN-γ and a small amount of IL-1β, as well as anti-inflammatory cytokine IL-10. Curcumin inhibits proinflammatory cytokines and decreases the ratio of proinflammatory and anti-inflammatory cytokines. Conclusion Curcumin can reduce the streptococcus suis induced splenocyte production of pro-inflammatory cytokines and play an anti-inflammatory effect.