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嘧啶核苷磷酸化酶是嘧啶核苷的分解、合成代谢中的一个重要酶,在肿瘤组织中的活性明显高于正常组织.人体内,乳腺肿瘤和胃肠道肿瘤中嘧啶核苷磷酸化酶的活性高于其他系统的肿瘤.嘧啶核苷磷酸化酶使抗代谢药脱氧氟尿苷(5’-DFUR)转化为5-FU,而发挥选择性的细胞毒作用.近来发现嘧啶核苷磷酸化酶与血小板源性内皮细胞生长因子是同一物质,具有血管增生作用.为了对嘧啶核苷磷酸化酶在乳癌组织中的表达与微血管密度的关系及氟铁龙的抗肿瘤活性进行探讨,为临床治疗提供理论基础.收集北京医科大学人民医院乳腺中心手术切除的40例乳腺癌标本(其中20例术前口服氟铁龙,20例对照),应用免疫组化染色,测定乳癌组织中嘧啶核苷磷酸化酶的表达和微血管密度.实验结果嘧啶核苷磷酸化酶的表达在应用氟铁龙后明显降低,和对照组比有显著性差异(P<0.01),乳癌分化越高,嘧啶核苷
Pyrimidine nucleoside phosphorylase is an important enzyme in the decomposition and anabolism of pyrimidine nucleosides. Its activity in tumor tissues is significantly higher than that in normal tissues. Human pyrimidine nucleoside phosphorylase in breast and gastrointestinal tumors The activity is higher than that of tumors in other systems. The pyrimidine nucleoside phosphorylase converts the antimetabolite deoxyfluorouridine (5’-DFUR) to 5-FU and exerts selective cytotoxicity. Recently, pyrimidine nucleoside phosphoric acid was found. The enzyme and platelet-derived endothelial cell growth factor are the same substances and have angiogenesis. To investigate the relationship between the expression of pyrimidine nucleoside phosphorylase in breast cancer tissue and microvessel density and the antitumor activity of fipronil Clinical treatment provides a theoretical basis. 40 breast cancer specimens (including 20 cases of oral ferricon before surgery and 20 controls) surgically removed at the breast center of the People’s Hospital of Beijing Medical University were collected. Immunohistochemical staining was used to determine the pyrimidine nucleus in breast cancer tissues. Expression of glucoside phosphorylase and microvessel density. The results showed that the expression of pyrimidine nucleoside phosphorylase was significantly lower after the application of fluoropyrimone, and there was a significant difference compared with the control group (P<0.01). The higher the pyrimidine nucleoside