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目的 :观察死胎多脏器组织中 HBV DNA表达情况 ,探讨通过产妇传播到死胎多脏器组织中的 HBV是否存在复制。方法 :采集 40例乙型肝炎产妇产下的死胎 ,常规尸检 ,取大脑、胸腺、心脏、肺、肝、脾、肾脏、脐带、胎盘组织 ,采用原位分子杂交法 ,检测其中的HBV DNA;回访婴母产前血清血 HBV的检测结果。结果 :死胎多脏器组织中 HBV DNA阳性率在 (胸腺、心脏、肺、脾、肾脏、脐带 )、(肝、胎盘 )、大脑三组间有统计学意义。在产妇HBV呈无复制、低复制、高复制状态下 ,心、肺、肾组织中 HBV DNA阳性率有统计学意义 ;大脑、胸腺、肝、脾、脐带、胎盘组织中 HBV DNA阳性率无统计学意义。结论 :HBV DNA对胎儿组织的嗜好与胎儿脏器种类有关 ;孕妇 HBV血清中 HBV标志与死胎大脑、胸腺、肝、脾、脐带、胎盘中 HBV DNA阳性率无关 ,与死胎心、肺、肾组织中 HBV DNA阳性率有关 ;死胎多脏器组织中存在 HBV复制
OBJECTIVE: To observe the expression of HBV DNA in multiple organ tissues of the stillbirth and to explore whether there is replication of HBV in the multiple organ tissues of the stillbirth through maternal transmission. Methods: Forty fetuses with stillbirth from hepatitis B were collected and routinely autopsy. The brain, thymus, heart, lung, liver, spleen, kidney, umbilical cord and placenta were collected and the HBV DNA was detected by in situ hybridization ; Visit the baby prenatal serum HBV test results. Results: The positive rates of HBV DNA in multiple organ tissues of stillbirth were statistically significant among three groups (thymus, heart, lung, spleen, kidney and umbilical cord), (liver, placenta) and brain. The positive rate of HBV DNA in heart, lung and kidney tissue was statistically significant in the condition of no replication, low replication and high replication of HBV in HBV-DNA. The positive rate of HBV DNA in brain, thymus, liver, spleen, umbilical cord and placenta was not statistically Significance of learning. Conclusion: The HBV DNA to fetus tissue is related to the type of fetal organs. HBV markers in pregnant women have nothing to do with HBV DNA positive rate in fetal brain, thymus, liver, spleen, umbilical cord and placenta, HBV DNA positive rate in the organization; stillbirth multiple organism HBV replication