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目的:研究附子粳米汤对脾阳虚大鼠疼痛模型血浆降钙素基因相关肽(CGRP)和血管紧张素II(AngII)的影响。方法:取Wister大鼠50只,随机分为5组,即空白对照组、模型对照组、附子粳米汤高、低剂量组(9.4,2.35 g.kg-1)和西药消炎痛对照组7.81 mg.kg-1,每组10只。除空白对照组正常饲养,其余各组大鼠造脾阳虚疼痛模型,除模型对照组和空白对照组给予生理盐水,药物组分别ig给予附子粳米汤和消炎痛干预,各组给药15 d后取血,制备血浆,用放免法测定各组血浆CGRP和AngII水平。结果:与空白对照组比较,模型对照组大鼠血浆CGRP含量明显降低(P<0.01),AngII含量明显升高(P<0.01);与模型对照组比较,附子粳米汤高、低剂量组、西药对照组大鼠血浆CGRP含量明显升高(P<0.01或P<0.05),AngII含量明显降低(P<0.01或P<0.05)。结论:附子粳米汤具有明显改善脾阳虚大鼠的疼痛症状,可调节血浆CGRP和AngII水平,说明该方镇痛作用可能与其调节胃肠肽类激素的水平有关。
Objective: To study the effect of aconite japonica rice soup on plasma calcitonin gene related peptide (CGRP) and angiotensin II (AngII) in pain model of spleen yang deficiency rats. Methods: Fifty Wister rats were randomly divided into five groups: blank control group, model control group, aconite japonica rice high and low dose group (9.4,2.35 g.kg-1) and western medicine indomethacin control group (7.81 mg kg-1, 10 in each group. Rats in the control group and control group were given physiological saline except the blank control group. The rats in the other groups were treated with aconite japonica rice soup and indomethacin for 15 days After the blood was drawn, the plasma was prepared and the levels of plasma CGRP and AngII in each group were determined by radioimmunoassay. Results: Compared with the blank control group, the content of CGRP in the model control group was significantly decreased (P <0.01) and the content of Angll was significantly increased (P <0.01). Compared with the model control group, Western medicine control group rats plasma CGRP levels were significantly increased (P <0.01 or P <0.05), AngII content was significantly lower (P <0.01 or P <0.05). Conclusion: The aconite japonica rice soup can significantly improve the pain symptoms of rats with spleen yang deficiency and regulate the levels of CGRP and AngII in plasma, indicating that the analgesic effect may be related to the regulation of gastrointestinal peptide hormones.