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建立了三重四级杆串联质谱(GC-MSMS)结合固相微萃取技术(SPME)检测IPA啤酒、麦汁及酒花萃取液中的果香硫醇的方法。采用100μm PDMS纤维,先将纤维插入装有15 m L样品及5 g Na Cl的20 m L顶空瓶中,在室温(25℃)萃取180 min。采用GC-MSMS多反应监测扫描(MRM)模式,果香硫醇在线性范围内其响应值与浓度有较好的线性关系,相关系数达到0.993以上。样品测得值相对标准偏差(RSD%)为4.2%~6.3%(n=5),回收率98.5%~101.4%(n=3)。实验了萃取纤维、Na Cl添加量、p H及萃取时间对果香硫醇测量值的影响。该方法可用于酒花萃取液、麦汁及啤酒的风味研究与产品质量控制及检测Cascade酒花萃取液及IPA麦汁与成品啤中果香硫醇的含量。
A method for the determination of fructose thiols in IPA beer, wort and hops extract by triple quadrupole tandem mass spectrometry (GC-MSMS) and solid-phase microextraction (SPME) was established. Using 100 μm PDMS fibers, the fibers were first inserted into a 20 mL headspace vial containing 15 mL samples and 5 g NaCI and extracted for 180 min at room temperature (25 ° C). Using GC-MSMS MRM (Scanning Electron Microscope), the response and concentration of fructose-thiol had a good linearity within the linear range, and the correlation coefficient reached above 0.993. The relative standard deviations (RSD%) of the samples were 4.2% ~ 6.3% (n = 5) and the recoveries were 98.5% ~ 101.4% (n = 3). The effects of extraction fiber, NaCl addition amount, p H and extraction time on the determination of fructose thiols were investigated. The method can be used for the flavor research and product quality control of hop extracts, wort and beer, and the detection of fructose thiols in Cascade hop extract and IPA wort and finished beer.