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用聚苯乙烯标样作为探针,对多种SEC柱的分离和扩展效应作同时的校准,扩展因子随探针分子量的增加而增大,增大的幅度与SEC填料的孔洞表面结构有关。大孔径多孔硅球的比表面积较低,孔洞表面比较平滑,溶质分子在孔洞中运动时阻碍粳少,高分子量聚苯乙烯(M?10~6)的扩展因子只比低分子量(M?10~3)的大2—3倍。交联聚苯乙烯型凝胶的比表面积较大,表面比较粗糙,高分子量探针的扩展因子要比低分子量的高出几十倍,显示溶质分子在孔洞中运动所受阻碍较大。后一现象可用有机交联凝胶永久性孔洞的骨架表面具有自由链端得到说明。
Using polystyrene standards as probes, the separation and spreading effects of various SEC columns were calibrated at the same time. The expansion factor increased with the increase of probe molecular weight, which was related to the pore surface structure of SEC fillers. The large specific surface area of porous silica balls is relatively low, the surface of pores is relatively smooth, and the solute molecules impede the formation of japonica when they move in the pores. The expansion factor of high molecular weight polystyrene (M 10 ~ 6) is only lower than that of low molecular weight ~ 3) big 2-3 times. Cross-linked polystyrene gel larger specific surface area, the surface rough, high-molecular-weight probe expansion factor than the low molecular weight higher than a few times, showing solute molecules in the hole in the movement of the greater obstacles. The latter phenomenon can be used organic cross-linked gel permanent hole skeleton surface has a free chain end to get instructions.