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目的研究承德产穿山龙药材的HPLC指纹图谱并测定薯蓣皂苷元的含量。方法选择10批承德不同产地的穿山龙,用Agilent Eclipse C18色谱柱,以水和乙腈为流动相,梯度洗脱,检测波长203 nm,柱温30℃,流速1.0 mL·min-1,分析时间90 min。结果不同产地的穿山龙药材中薯蓣皂苷元的含量差别较小,指纹图谱共标示出13个共有峰。利用中药色谱指纹图谱相似度评价系统研究版(2004A)软件,以夹角余弦为测度,测得10批穿山龙药材的指纹图谱相似度为0.90～1.00,说明各批穿山龙药材之间既有较好的相关性,又有区别。结论 HPLC指纹图谱可整体显示穿山龙药材的特征,为穿山龙的质量控制提供了有效手段。
Objective To study the HPLC fingerprinting of Chengde Longshan medicinal herbs and determine the content of diosgenin. Methods Ten batches of Chimonanthus aspartaton from different areas of Chengde were selected and analyzed by Agilent Eclipse C18 column with gradient elution with water and acetonitrile at the wavelength of 203 nm with a column temperature of 30 ℃ and a flow rate of 1.0 mL · min -1 min. Results The content of diosgenin in S. aureus from different habitats was slightly different. The fingerprint showed 13 common peaks. The similarity of the fingerprints of 10 medicinal plants of Radix Aconiti Lateralis from 0.90 to 1.00 was measured by cosine of cosine, using the software of similarity evaluation system of chromatographic fingerprints of Chinese traditional medicine (2004A). The relevance, there are differences. Conclusion HPLC fingerprinting can display the characteristics of the Chinese medicinal herbs, which provides an effective method for the quality control of the Chinese medicinal plants.