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建立了一种直链淀粉键合手性固定相拆分dracocephins A对映异构体的方法。使用Chiralpak IA柱(4.6 mm×250 mm,5μm),流动相为正己烷-乙酸乙酯(10∶90),流速1.0 mL·min-1,检测波长290 nm,柱温为室温。所建立的方法能有效实现dracocephins A两对对映异构体的同时拆分,通过CD检测器检测结合与单一构型异构体的比对,可以确定4种异构体出峰顺序。直链淀粉键合手性固定相可以用于dracocephins A对映异构体的手性分离。
A method was established for the resolution of dracocephins A enantiomers using amylose-bonded chiral stationary phases. Chiralpak IA column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was n-hexane-ethyl acetate (10:90) and the flow rate was 1.0 mL · min-1. The detection wavelength was 290 nm and the column temperature was room temperature. The established method can effectively achieve the simultaneous resolution of two enantiomers of dracocephins A. The peak order of the four isomers can be determined by detecting the alignment of the binding and single configuration isomers by CD detector. Amylose-bonded chiral stationary phases can be used for the chiral separation of dracocephins A enantiomers.