论文部分内容阅读
目的:探讨肺动脉高压新生儿外周血中肿瘤坏死因子-α(TNF-α)、血管生成素-1(ANG-1)和白细胞介素-8(IL-8)细胞因子的变化水平与疾病的关系。方法:以临床确诊的42例肺动脉高压患儿为研究对象,与16例正常新生儿进行比较,采用Elisa方法检测血清中TNF-α、ANG-1和IL-8蛋白含量。采用实时荧光定量PCR法检测TNF-α、ANG-1和IL-8mRNA水平。结果:与健康新生儿相比较,肺动脉高压患儿外周血ANG-1的蛋白含量及ANG-1mRNA表达水平明显降低(P<0.01);TNF-α、IL-8的蛋白含量及TNF-α、IL-8的mRNA表达水平明显升高(P<0.01)。结论:TNF-α、ANG-1和IL-8参与肺动脉高压的发病过程。
Objective: To investigate the changes of cytokine levels of tumor necrosis factor-α (TNF-α), angiopoietin-1 (ANG-1) and interleukin-8 (IL-8) in peripheral blood of newborns with pulmonary hypertension relationship. Methods: Forty-two children with pulmonary hypertension were enrolled in this study. The serum levels of TNF-α, ANG-1 and IL-8 were detected by Elisa method in 16 normal newborns. The levels of TNF-α, ANG-1 and IL-8 mRNA were detected by real-time fluorescence quantitative PCR. RESULTS: Compared with healthy newborns, the protein levels of ANG-1 and the expression of ANG-1 mRNA in peripheral blood of children with pulmonary hypertension were significantly lower than those of healthy newborns (P <0.01); The protein levels of TNF-α and IL- IL-8 mRNA expression was significantly increased (P <0.01). Conclusion: TNF-α, ANG-1 and IL-8 are involved in the pathogenesis of pulmonary hypertension.