We developed a method for in vitro regeneration of Garcinia xanthochymus(yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant(WP) medium supplemented with cytokinins.An average of 11 shoots per explant were regenerated from mature seed segments on WP medium containing 20 μM 6-benzylaminopurine. Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meristemoids, subsequently leading to the formation of shoot buds.Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or a-naphthalene acetic acid. Plantlets were transplanted to pots containing soil: compost(1:1) and survival rate was 90%. We developed a method for in vitro regeneration of matured seed segments. Garlic xanthochymus (yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An average of 11 shoots per explant were regenerated from mature seed segments on WP Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meristemoids, subsequently leading to the formation of shoot buds. Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or a-naphthalene acetic acid. Plantlets were transplanted to pots containing soil: compost (1: 1) and survival rate was 90%.