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目的:为证明膀胱二聚体Cajal间质细胞(interstitial cells of Cajal,ICC)发挥起搏作用提供超微结构和功能学证据。方法:电子显微镜下鉴定组织和培养的豚鼠膀胱二聚体ICC并观察ICC之间的超微联系;体外分离、培养豚鼠膀胱ICC,运用细胞膜片钳技术观察ATP(100μmol/L)刺激下二聚体ICC和单体ICC的兴奋性差别。结果:二聚体ICC中两个ICC细胞胞体紧密相连,细胞之间的间隙小于20 nm,存在大量缝隙连接,局部细胞膜融合,形成联胞体。在ATP(100μmol/L)的刺激下体外培养的二聚体ICC的内向电流幅度为286.9±26.4 pA,显著大于单体ICC的内向电流幅度163.8±18.6 pA(P<0.01)。结论:二聚体ICC具有形成高兴奋起搏电流的超微结构基础,高兴奋性的二聚体ICC可能才是逼尿肌的起搏细胞。
Objective: To provide ultrastructural and functional evidence for the pacing effect of interstitial cells of Cajal (ICC). Methods: ICC of guinea pig bladder tissue and cultured guinea pig were identified by electron microscopy and ultrastructure of ICC were observed. ICC of guinea pig bladder was isolated and cultured in vitro. The effect of ATP (100μmol / L) Excitability differences between ICC and ICC. Results: Two ICC cells in ICC were closely linked to each other. The gap between the two ICCs was less than 20 nm. There were a large number of gap junction and local cell membrane fusion to form syncytia. The inward current amplitude of dimeric ICC incubated with ATP (100μmol / L) was 286.9 ± 26.4 pA, significantly higher than that of monomeric ICC (163.8 ± 18.6 pA, P <0.01). CONCLUSIONS: The dimeric ICC has the ultrastructural basis for the formation of an arousal pacing current, and the high excitatory dimeric ICC may be the detrusor pacemaker.