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目的:研究小鼠骨髓间充质干细胞对胰腺癌的聚集作用。方法:体外分离培养纯化绿色荧光蛋白转基因小鼠骨髓间充质干细胞,通过Transwell共培养体系观察骨髓间充质干细胞向胰腺癌细胞PNAC-1的迁移;建立裸鼠胰腺癌移植瘤模型,经尾静脉回输骨髓间充质干细胞至荷瘤鼠体内,荧光显微镜动态观察肿瘤组织及其他脏器组织中骨髓间充质干细胞的分布及含量。结果:通过贴壁培养获得小鼠骨髓间充质干细胞,流式细胞仪检测证实,CD44+CD45-、CD90+CD45-细胞均占细胞总数90%以上;Transwell共培养提示骨髓间充质干细胞向胰腺癌细胞PANC-1迁移,且随着肿瘤细胞数的增多,细胞迁移的数量增多(P<0.05);体内实验证实,骨髓间充质干细胞向胰腺癌组织特异性地靶向聚集,10 d内随着时间的延长骨髓间充质干细胞在胰腺癌组织中逐渐增多(P<0.05),10 d后增加不明显。结论:小鼠骨髓间充质干细胞体外向胰腺癌细胞迁移,体内向胰腺癌组织靶向聚集。
Objective: To study the aggregation of mouse bone marrow mesenchymal stem cells on pancreatic cancer. Methods: BMSCs were isolated and purified from green fluorescent protein transgenic mice in vitro. Transplantation of BMSCs into PNAC-1 cells was performed by Transwell co-culture system. The model of transplanted pancreatic cancer in nude mice was established. Intravenous bone marrow-derived mesenchymal stem cells were transplanted into tumor-bearing mice, and the distribution and content of bone marrow-derived mesenchymal stem cells in tumor tissue and other organs were observed dynamically by fluorescence microscopy. Results: Mice bone marrow mesenchymal stem cells were obtained by adherent culture. Flow cytometry confirmed that CD44 + CD45- and CD90 + CD45- cells accounted for more than 90% of the total number of cells. Transwell co-culture showed that bone marrow mesenchymal stem cells Pancreatic cancer cells PANC-1 migrated, and with the increase of the number of tumor cells, the number of cell migration increased (P <0.05); in vivo experiments showed that bone marrow-derived mesenchymal stem cells targeted specifically to pancreatic cancer tissue aggregation, 10 d Bone marrow-derived mesenchymal stem cells increased gradually with time (P <0.05), but did not increase significantly after 10 days. CONCLUSION: Mouse bone marrow mesenchymal stem cells migrate to pancreatic cancer cells in vitro and target to pancreatic cancer tissues in vivo.