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为评价枣庄某地区生活饮用水和水源水中有机提取物(organic extract substance,OES)的遗传毒性,于2011年10月采集该地区某水厂的水源水及出厂水水样,并提取OES。将48只健康10周龄清洁级雄性昆明小鼠随机分为5组,分为阴性对照(5%DMSO)组及0.2、1L/ml水源水和出厂水OES染毒组,采用灌胃方式进行染毒;并设阳性对照[10 mg/ml环磷酰胺(CP),分别于实验第40、42天采用腹腔注射方式染毒]组,每组8只。染毒容量为10ml/kg,每天1次,连续染毒6周。采用胞质分裂阻滞微核试验和慧星试验分别检测染色体和DNA的损伤情况。结果显示,与阴性对照组和相同水样低剂量组比较,1L/ml水源水和出厂水OES染毒组小鼠外周血淋巴细胞的双核细胞率和核分裂指数均较低(P<0.05);DNA的尾长、头尾光密度比及Olive尾矩均较高(P<0.05);而小鼠外周血淋巴细胞的微核率仅略有增高(P>0.05),未见显著性差异。提示水源水和饮用水中OES皆具有一定的致DNA损伤能力,并抑制细胞分裂,未发现引起明显的染色体损伤。
In order to evaluate the genotoxicity of organic extract substance (OES) in drinking water and source water in a certain area of Zaozhuang, water source water and manufactured water sample of a water plant in the area were collected in October 2011 and OES was extracted. Forty-eight healthy Kunming mice, aged 10 weeks, were randomly divided into 5 groups and divided into negative control group (5% DMSO) and 0.2,1 L / ml water source water and factory water OES exposure group (10 mg / ml cyclophosphamide (CP), respectively, on the 40th and 42nd days of the experiment by intraperitoneal injection group], with 8 rats in each group. Exposure capacity of 10ml / kg, 1 day, continuous exposure to 6 weeks. Cytokinesis and micronucleus test were used to detect chromosomal and DNA damage respectively. The results showed that compared with the negative control group and the same low water sample group, the peripheral blood mononuclear cells and mitotic index of peripheral blood lymphocytes were lower in 1L / ml water source and ex-factory water OES groups (P <0.05); DNA tail length, head and tail optical density ratio and Olive tail moment were higher (P <0.05). However, the micronucleus rate of peripheral blood lymphocytes in mice increased only slightly (P> 0.05). No significant difference was found. Tip OES in drinking water and drinking water have a certain ability to cause DNA damage and inhibit cell division, found no significant chromosomal damage.