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目的:探讨canstatin基因治疗对人卵巢癌移植瘤的影响。方法:18只裸鼠皮下接种人卵巢癌HO8910PM细胞制备人卵巢癌移植瘤模型,当移植瘤体积达到50mm3时,随机分3组治疗,分别注射腺病毒介导的canstatin基因(Ad-can)、Ad-GFP和PBS,2次瘤内多点注射,间隔72h,每次4×1010pfu。治疗期间每周2次用游标卡尺测量皮下移植瘤的长径和短径并计算移植瘤体积;治疗30d后处死裸鼠,切取肿瘤检测肿瘤组织中Caspase-3、Flk-1蛋白的表达。结果:Ad-can治疗组肿瘤体积小于其余2组(P<0.05)。3组肿瘤组织中均有坏死,Ad-can治疗组坏死明显。Ad-can治疗组Caspase-3蛋白表达高于Ad-GFP组和PBS组(P<0.05),Flk-1蛋白的表达低于其他2组(P<0.05)。结论:人canstatin基因可能通过诱导肿瘤细胞凋亡、抑制肿瘤的血管生成,从而抑制人卵巢癌细胞的生长。
Objective: To investigate the effect of canstatin gene therapy on human ovarian cancer xenografts. Methods: Twenty-eight nude mice were inoculated subcutaneously with human ovarian cancer cell line HO8910PM to prepare a human ovarian cancer xenograft model. When the transplanted tumor volume reached 50 mm 3, the mice were randomly divided into 3 groups. The adenovirus-mediated canstatin gene (Ad-can) Ad-GFP and PBS, two injections intratumoral, interval 72h, each 4 × 1010pfu. During the treatment, the long and short diameters of the subcutaneously transplanted tumor were measured by vernier caliper twice a week and the volume of the transplanted tumor was calculated. After 30 days of treatment, the nude mice were sacrificed and the tumors were excised to detect the expression of Caspase-3 and Flk-1. Results: The tumor volume in Ad-can treatment group was smaller than the other two groups (P <0.05). Three groups of tumor necrosis, Ad-can treatment group was significantly necrosis. Caspase-3 protein expression in Ad-can treatment group was higher than that in Ad-GFP group and PBS group (P <0.05), and Flk-1 protein expression was lower than the other two groups (P <0.05). Conclusion: The human canstatin gene may inhibit the growth of human ovarian cancer cells by inducing tumor cell apoptosis and inhibiting tumor angiogenesis.