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目的：观察体外培养的人胚鼻咽上皮（HENE）被EB病毒（EBV）感染后，c－myc和p53表达的变化，以探讨EBV和这些癌基因在鼻咽癌发生发展中可能起的作用。方法：体外培养的人胚鼻咽上皮用EBV或EBV加TPA感染（简称E和ET组），分别用抗补体免疫荧光法（ACIF）和磁性磷酸酶抗碱性磷酸酶法（APAAP）检测EBV核抗原（EBNA）及癌基因c－myc和p53的表达。结果；体外培养10例入胚鼻咽上皮细胞波EBV感染后，EBNA全部阳性，c－myc、p53总表达和p53突变型的表达明显增加，E组阳性细胞分别为（39．4±16．8）％、（18．6±11．0）％和（11．6±2．0）％，ET组为（458±16．9）％、（7.4±2.4）％和（8．0±3．5）％，与不受病毒感染的对照组〔（0．0±0．0）％、（3．8±2．3）％和（3．6±2．1）％〕的差别，除ET组p53总表达外均有显著的统计学意义（P＜0．01或P＜0．05）。结论：提示EBV感染鼻咽上皮，可导致某些癌基因的活化和表达增强，可能在鼻咽癌的发生和发展中起一定作用。
OBJECTIVE: To observe the changes of c-myc and p53 expression in EBV-infected human embryonic nasopharyngeal epithelium (HENE) cultured in vitro to explore the possible role of EBV and these oncogenes in the development of nasopharyngeal carcinoma . Methods: Human embryonic nasopharyngeal epithelial cells cultured in vitro were infected with EBV or EBV plus TPA (E and ET for short), and the levels of EBV (P <0.05) were detected by anti-complement immunofluorescence (ACIF) and alkaline phosphatase (APAAP) Nuclear antigen (EBNA) and oncogene c-myc and p53 expression. Results EBV infection of EBs in 10 cases of nasopharyngeal epithelial cells in vitro showed that all EBNA positive cells, c-myc, p53 and p53 mutant cells were significantly increased. The positive cells in group E were (39.4 ± 16) 8, 18.6 ± 11.0% and 11.6 ± 2.0% in ET group and 458 ± 16.9%, 7.4 ± 2.4% and 8.0 ± 3.5)%, which was significantly lower than that of the control group [(0.0 ± 0.0)%, (3.8 ± 2.3)% and (3.6 ± 2.1)%] , Except for the total expression of p53 in ET group, there was significant statistical significance (P <0.01 or P <0.05). Conclusion: EBV infection in nasopharyngeal epithelium may lead to the activation and expression of some oncogenes, which may play a role in the occurrence and development of nasopharyngeal carcinoma.