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目的:观察体外培养的人胚鼻咽上皮(HENE)被EB病毒(EBV)感染后,c-myc和p53表达的变化,以探讨EBV和这些癌基因在鼻咽癌发生发展中可能起的作用。方法:体外培养的人胚鼻咽上皮用EBV或EBV加TPA感染(简称E和ET组),分别用抗补体免疫荧光法(ACIF)和磁性磷酸酶抗碱性磷酸酶法(APAAP)检测EBV核抗原(EBNA)及癌基因c-myc和p53的表达。结果;体外培养10例入胚鼻咽上皮细胞波EBV感染后,EBNA全部阳性,c-myc、p53总表达和p53突变型的表达明显增加,E组阳性细胞分别为(39.4±16.8)%、(18.6±11.0)%和(11.6±2.0)%,ET组为(458±16.9)%、(7.4±2.4)%和(8.0±3.5)%,与不受病毒感染的对照组〔(0.0±0.0)%、(3.8±2.3)%和(3.6±2.1)%〕的差别,除ET组p53总表达外均有显著的统计学意义(P<0.01或P<0.05)。结论:提示EBV感染鼻咽上皮,可导致某些癌基因的活化和表达增强,可能在鼻咽癌的发生和发展中起一定作用。
OBJECTIVE: To observe the changes of c-myc and p53 expression in EBV-infected human embryonic nasopharyngeal epithelium (HENE) cultured in vitro to explore the possible role of EBV and these oncogenes in the development of nasopharyngeal carcinoma . Methods: Human embryonic nasopharyngeal epithelial cells cultured in vitro were infected with EBV or EBV plus TPA (E and ET for short), and the levels of EBV (P <0.05) were detected by anti-complement immunofluorescence (ACIF) and alkaline phosphatase (APAAP) Nuclear antigen (EBNA) and oncogene c-myc and p53 expression. Results EBV infection of EBs in 10 cases of nasopharyngeal epithelial cells in vitro showed that all EBNA positive cells, c-myc, p53 and p53 mutant cells were significantly increased. The positive cells in group E were (39.4 ± 16) 8, 18.6 ± 11.0% and 11.6 ± 2.0% in ET group and 458 ± 16.9%, 7.4 ± 2.4% and 8.0 ± 3.5)%, which was significantly lower than that of the control group [(0.0 ± 0.0)%, (3.8 ± 2.3)% and (3.6 ± 2.1)%] , Except for the total expression of p53 in ET group, there was significant statistical significance (P <0.01 or P <0.05). Conclusion: EBV infection in nasopharyngeal epithelium may lead to the activation and expression of some oncogenes, which may play a role in the occurrence and development of nasopharyngeal carcinoma.