目的：探讨Ｇｑ蛋白介导血管活性多肽对ｖＳＭＣＤＮＡ合成及增殖细胞核抗原（ＰＣＮＡ）蛋白表达的影响。方法：用硫代修饰的Ｇαｑ／１１亚基反义寡聚脱氧核苷酸（Ｇαｑ／１１ＡＳ－ＯＤＮｓ），以６μｍｏｌ／Ｌ的浓度加入含１０－７ｍｏｌ·Ｌ－１ＥＴ－１刺激无血清ＤＭＥＭ培养基中体外培养ｖＳＭＣ。用３Ｈ－ＴｄＲ掺入法测定ｖＳＭＣＤＮＡ合成、免疫细胞化学技术检测ｖＳＭＣＰＣＮＡ蛋白表达。结果：Ｇαｑ／１１ＡＳ－ＯＤＮｓ可明显抑制ｖＳＭＣＤＮＡ的合成，在１２ｈ，２４ｈ时抑制率分别为８４２％和８５３％；Ｇαｑ／１１ＡＳ－ＯＤＮｓ亦明显降低ｖＳＭＣＰＣＮＡ蛋白的表达。而相同浓度的正义Ｇαｑ／１１ＯＤＮｓ只呈现少量抑制作用。结论：本实验提示Ｇαｑ／１１ＡＳ－ＯＤＮｓ有可能进一步应用于由血管活性多肽刺激引起的ｖＳＭＣ增生性疾病如经皮冠脉腔内血管成形术（ＰＴＣＡ）后再狭窄的防治的研究 Objective: To investigate the effects of Gq-mediated vasoactive peptides on the synthesis of vSMCDNA and the expression of proliferating cell nuclear antigen (PCNA). Methods: Thi-modified Gαq / 11 subunit antisense oligodeoxynucleotide (Gαq / 11AS-ODNs) was added to the culture medium containing 10-7mol·L-1ET-1 stimulated serum-free DMEM at a concentration of 6μmol / L In vitro vSMC was cultured in vitro. The synthesis of vSMCDNA was determined by 3H-TdR incorporation and the expression of vSMCPCNA protein was detected by immunocytochemistry. Results: Gαq / 11AS-ODNs could significantly inhibit the synthesis of vSMCDNA. The inhibition rates at 84h and 85h were 842% and 853% at 12h and 24h, respectively. Gαq / 11AS-ODNs also significantly decreased the expression of vSMCPCNA. The same concentration of sense Gαq / 11ODNs showed only a small amount of inhibition. CONCLUSIONS: This experiment suggests that Gαq / 11AS-ODNs may be further used in the prevention and treatment of vSMC proliferative diseases such as restenosis after percutaneous transluminal coronary angioplasty (PTCA) induced by vasoactive peptides