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一、前言液相色谱分离过程中柱壁效应对柱效的影响已有详细讨论。如何消除或减小这种影响,是色谱工作者经常探讨的一个问题。如果将样品置于色谱柱进口填料床层的中心,成为一个点,则样品从柱中通过时就
I. Introduction The effect of columnar wall effect on column efficiency during liquid chromatographic separation has been discussed in detail. How to eliminate or reduce this effect is one of the most frequently asked by chromatographers. If the sample is placed at the center of the column’s inlet packed bed and becomes a point, the sample passes through the column