论文部分内容阅读
目的探讨兔髂动脉内膜损伤和再损伤后,TPO,EPO和GM-CSF的变化及意义。方法建立狭窄与再狭窄动物模型,模仿PTCA血管内膜损伤过程,观察TPO、EPO和GM-CSF促进内膜增殖的作用。结果兔髂动脉损伤后第7天TPO较术前明显升高,由65.17±10.19mg/ml增至131.78±52.36mg/ml(P<0.05),再损伤后第7天再次升高,由45.37±17.20mg/ml增至126.85±13.85mg/ml(P<0.05)。EPO仅于再损伤后1小时较术前明显升高,由112.36±14.80mU/ml增至168.70±28.86mU/ml(P<0.05)。GM-CSF在损伤后1小时较术前明显升高,由1198.67±140.63pg/ml增至1682.75±248.12pg/ml(P<0.05),损伤后第7天降至术前水平(P<0.05);再损伤后1小时再次升高至1441.33±101.64pg/ml(P<0.05),再损伤后第7天再降至术前水平(P<0.05)。结论血管内膜损伤和再损伤时,TPO,EPO和GM-CSF分泌增加,并促进血小板增生和加强其功能,它们可能是冠状动脉介入治疗后晚期再狭窄的重要因素之一。
Objective To investigate the changes and significance of TPO, EPO and GM-CSF in rabbit iliac artery after intima injury and re-injury. Methods The animal models of stenosis and restenosis were established to mimic the intimal hyperplasia of PTCA. The effects of TPO, EPO and GM-CSF on intima proliferation were observed. Results TPO was significantly increased at day 7 after iliac artery injury from 65.17 ± 10.19mg / ml to 131.78 ± 52.36mg / ml (P <0.05), and then increased again on the 7th day after injury. From 45.37 ± 17.20 mg / ml to 126.85 ± 13.85 mg / ml (P <0.05). The level of EPO increased significantly from 112.36 ± 14.80mU / ml to 168.70 ± 28.86mU / ml (P <0.05) only 1 hour after re-injury compared with that before operation. The level of GM-CSF was significantly increased at 1 hour after injury from 1198.67 ± 140.63pg / ml to 1682.75 ± 248.12pg / ml (P <0.05), and decreased to preoperative level on the 7th day after injury (P <0.05 ) Again increased to 1441.33 ± 101.64pg / ml 1 hour after injury (P <0.05), and then dropped to preoperative level on the 7th day after injury (P <0.05). Conclusions Increased secretion of TPO, EPO and GM-CSF, and promotion of platelet proliferation and enhancement of function during intimal injury and reperfusion may be one of the important factors of late restenosis after coronary intervention.