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目的:建立高效液相色谱法测定复方氯霉素阴道泡腾片中己烯雌酚的含量。方法:色谱柱为DIONEX C_(18)(250mm×4.6mm,5μm);流动相为甲醇-水(75:25);流速为1.0 ml·min~(-1);检测波长为239nm;进样量为50μl。结果:己烯雌酚的线性范围为1.1～8.6μg·ml~(-1)(r=0.9999);平均回收率为100.8%(RSD=1.9%)。结论:本方法结果准确可靠,可作为复方氯霉素阴道泡腾片中己烯雌酚的含量测定方法。
Objective: To establish a method for the determination of diethylstibestrol in compound chloramphenicol vaginal effervescent tablets by high performance liquid chromatography. METHODS: The chromatographic column was DIONEX C 18 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (75:25). The flow rate was 1.0 ml · min -1. The detection wavelength was 239 nm. The amount is 50 μl. Results: The linear range of diethylstilbestrol was 1.1 ~ 8.6μg · ml -1 (r = 0.9999). The average recovery was 100.8% (RSD = 1.9%). Conclusion: The method is accurate and reliable and can be used as a method for the determination of diethylstilbestrol in compound chloramphenicol vaginal effervescent tablets.