吸光光度法测定微量钙的早期工作,E.B.Sandell 已有评述。近二十年来,分析化学迅速发展,各种仪器分析方法如发射光谱法、原子吸收分光光度法、离子选择电极法、X 荧光分析法以及中子活化分析法均可用于微量钙的测定,然而仪器分析法不仅需要昂贵的设备,而且由于化学干扰严重等原因,在测定复杂试样中微量钙时,仍需要进行烦琐的分离,故优越性不显著。吸光光度法具有简便、快速、灵敏度高以及成本较低等特点,在整个分析领域中,至今仍不失其重要地位,在微量钙的测定方面,亦颇有进展。概括起来可归纳为两点:其一,利用原有试剂,改变显色条件(介质、酸度或萃取酸度),从 The early work on the determination of trace calcium by spectrophotometry has been reviewed by E. B. Sandell. Nearly two decades, the rapid development of analytical chemistry, a variety of instrumental analysis methods such as emission spectrometry, atomic absorption spectrophotometry, ion-selective electrode method, X fluorescence analysis and neutron activation analysis can be used for the determination of trace calcium, however Instrumental analysis not only requires expensive equipment, but also due to serious chemical interference and other reasons, the determination of trace calcium in complex samples, still need to be cumbersome separation, so the advantage is not significant. Absorbance spectrophotometry is simple, rapid, high sensitivity and low cost characteristics, in the entire field of analysis, it still does not lose its important position in the determination of trace calcium has also made considerable progress. To sum up can be summed up in two points: First, the use of the original reagent, change the color conditions (medium, acidity or extraction acidity), from