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目的研究系统性红斑狼疮(SLE)患者外周血Vα24Vβ11自然杀伤T细胞(NKT)细胞数量变化和功能状态在致病中的作用。方法采用流式细胞仪检测32例SLE患者和30名正常对照组Vα24Vβ11NKT细胞数量以及体外活化前后表达血细胞分化抗原(CD)69和白细胞介素4(IL4)、Ⅱ型干扰素(IFNγ)的水平。结果SLE患者外周血Vα24Vβ11NKT细胞数量(044%±025%)低于正常对照组(107%±023%,P<001),NKT细胞体外活化前CD69表达为526%±212%,正常对照组为1147%±286%(P<005);活化后表达CD69为5661%±047%,正常对照组为9671%±033%(P<001)。SLE患者NKT细胞活化前其胞浆内IL4含量为3246±549pg/ml,正常对照组为1221±331pg/ml,活化后其胞浆内IL4含量为4838±853pg/ml,正常对照为2693±684pg/ml(均P<005),而IFNγ含量活化后为1932±645pg/ml,正常对照组为3365±1191pg/ml(P<005)。结论SLE发病可能与NKT细胞数量的低下及功能严重失调相关。
Objective To study the changes of the number of Vα24Vβ11 natural killer T cells (NKT) in peripheral blood of patients with systemic lupus erythematosus (SLE) and the role of functional status in pathogenicity. Methods The number of Vα24Vβ11NKT cells in 32 patients with SLE and 30 normal controls were measured by flow cytometry. The levels of CD 69 and IL-4 and IFN-γ before and after activation in vitro were measured. . Results The number of Vα24Vβ11NKT cells (044% ± 025%) in peripheral blood of SLE patients was lower than that of the normal controls (107% ± 023%, P <001). The expression of CD69 in NKT cells was 526% ± 212% 1147% ± 286% (P <005). The expression of CD69 was 5661% ± 047% after activation and 9671% ± 033% in the normal control group (P <0.001). The level of IL4 in cytoplasm of SLE patients before NKT cell activation was 3246 ± 549pg / ml, that of normal control group was 1221 ± 331pg / ml, and the cytoplasm IL4 content was 4838 ± 853pg / ml after activation. The normal control was 2693 ± 684pg / ml (all P <005), while IFNγ content was 1932 ± 645pg / ml after activation, and 3365 ± 1191pg / ml in normal control group (P <005). Conclusion The pathogenesis of SLE may be related to the low number of NKT cells and severe dysfunction.