目的建立工作场所空气中双氰胺的滤膜采集高效液相色谱测定方法。方法滤膜采集空气中双氰胺,用水洗脱,甲醇∶水(1∶99)为流动相,1ml/min的流速经C18柱(250.0mm×4.6mm×5.0μm,柱温30℃)分离,于213nm处检测。结果标准曲线线性范围0～10mg/L,回归方程y=411805x+14573,相关系数(r)=0.9999,检出限36μg/L,以采集75L空气样品计,最低检出浓度4.8μg/m3。方法的相对标准偏差小于5%,洗脱效率为96.5%～103.1%,样品在室温下至少可稳定14d。结论本法可应用于工作场所空气中双氰胺浓度的检测。 Objective To establish a method for the determination of dicyandiamide in workplace air by high performance liquid chromatography. Methods Dianhydrochloride in air was collected by membrane filtration. The membrane was eluted with water. The mobile phase consisted of methanol: water (1:99) and the flow rate was 1 ml / min and was separated by C18 column (250.0 mm × 4.6 mm × 5.0 μm, column temperature 30 ℃) At 213 nm. Results The linear range of the standard curve was 0-10 mg / L. The regression equation was y = 411805x + 14573. The correlation coefficient (r) was 0.9999. The detection limit was 36 μg / L. The minimum detectable concentration was 4.8 μg / m3. The relative standard deviation of the method was less than 5% and the elution efficiency was 96.5% ~ 103.1%. The sample was stable for at least 14 days at room temperature. Conclusion This method can be applied to the detection of dicyandiamide concentration in the air of workplace.