论文部分内容阅读
目的探讨对氧磷酶155Met/Leu(paraoxonase1,PON155Met/Leu)、对氧磷酶2148Ala/Gly(PON2148Ala/Gly)基因多态性与冠状动脉粥样硬化性心脏病(简称冠心病)、血浆对氧磷酶(paraoxonase,PON)、总超氧化物歧化酶(totalsuperoxidedismutase,T SOD)活性以及丙二醛(maleicdialdehyde,MDA)浓度的关系。方法采用聚合酶链反应-限制性片段长度多态性方法检测262例冠心病患者和100名对照的PON155Met/Leu、PON2148Ala/Gly基因多态性,采用比色法测定血浆PON、T SOD活性以及MDA浓度。结果与对照比较,冠心病患者的血浆PON[(349.27±138.36)nmol/min·mLvs.(454.75±166.00)nmol/min·mL,P<0.01]、T SOD[(23.61±16.51)U/mLvs.(44.01±22.68)U/mL,P<0.01]活性明显降低,MDA浓度显著增高[(2.47±0.73)nmol/mLvs.(2.15±0.55)nmol/mL,P<0.01];冠心病患者的PON155LM杂合子基因型(24.8%vs.1.4%,P<0.01)、M等位基因频率(12.4%vs.0.5%,P=0.001),PON2148GG纯合子基因型和AG杂合子基因型(11.8%vs.5.0%和48.1%vs.24.0%,P<0.01)、G等位基因频率(36.0%vs.17.0%,P<0.01)较对照组明显增高;PON155LM杂合子基因型的PON和T SOD活性较LL纯合子基因型明显降低(P<0.01和P<0.05);PON2148GG基因型和AG基因型的PON活性较AA基因型明显降低(P<0.01);Logistic回归分析显示PON155LM杂合子基因型、M等位基因、PON2148GG/AG基因型、G等位基因是冠心病的危险因子。结论冠心病患者的血浆PON和T SOD活性明显降低,MDA浓度显著增高;PON155Met/Leu的LM基因型和M等位基因、PON2148Ala/Gly的GG/AG基因型和G等位基因是冠心病的危险因子,并且与其他基因型相比,这些基因型患者的血浆PON活性降低。
Objective To investigate the relationship between polymorphism of paraoxonase gene 2148Ala / Gly (PON2148Ala / Gly) and coronary atherosclerotic heart disease (PON), total superoxide dismutase (T SOD) activity and the concentration of maleicdialdehyde (MDA). Methods The polymorphisms of PON155Met / Leu and PON2148Ala / Gly in 262 patients with CHD and 100 controls were detected by polymerase chain reaction - restriction fragment length polymorphism (PCR - RFLP). The plasma PON and T SOD activities were measured by colorimetry MDA concentration. Results Compared with the control group, the plasma levels of PON in patients with coronary heart disease (349.27 ± 138.36 nmol / min · mL vs 454.75 ± 166.00 nmol / min · mL, P <0.01) and T SOD (23.61 ± 16.51) U / mLvs (44.01 ± 22.68) U / mL, P <0.01], and the MDA level was significantly higher in patients with coronary heart disease (2.47 ± 0.73 nmol / mL vs 2.15 ± 0.55 nmol / mL, P <0.01) PON155LM heterozygous genotype (24.8% vs.1.4%, P <0.01), M allele frequency (12.4% vs.0.5%, P = 0.001), PON2148GG homozygous genotype and AG heterozygous genotype (11.8% P <0.05). The frequencies of G allele (36.0% vs.17.0%, P <0.01) were significantly higher than those in control group. PON and T SOD of PON155LM heterozygote (P <0.01 and P <0.05). The PON activity of PON2148GG genotype and AG genotype was significantly lower than that of AA genotype (P <0.01). Logistic regression analysis showed that PON155LM heterozygous genotype , M allele, PON2148GG / AG genotype, G allele is a risk factor for coronary heart disease. Conclusions Plasma PON and T SOD activity in patients with coronary heart disease were significantly lower and MDA content was significantly higher. The LM genotype and M allele of PON155Met / Leu, the GG / AG genotype and G allele of PON2148Ala / Gly were of coronary heart disease Risk factor, and plasma PON activity is reduced in these genotype patients compared to other genotypes.