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运用硅胶、MPLC和制备HPLC等柱色谱方法对蝉翼藤根的化学成分进行分离,并通过理化性质和MS、NMR波谱数据进行结构鉴定。从蝉翼藤根的95%乙醇提取物中分离鉴定了9个化合物,包括5个木质素苷acernikol-4″-O-β-D-glucopyranoside(1),(7R,8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside(2),(7R,8S)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside(3),(7R,8S)-dihydrodehydrodiconiferyl alcohol 9’-O-β-D-glucopyranoside(4),(7R,8S)-5-methoxydihydrodehy drodiconiferyl alcohol 4-O-β-D-glucopyranoside(5);3个蔗糖酯3,6’-O-diferuloylsucrose(6),3-O-feruloyl-6’-Osinapoylsucrose(7),sibricose A5(8);1个有机酸酯mehyl ferulate(9)。化合物1~5,8,9为首次从蝉翼藤属中分离得到。采用MTT法测定了化合物对肺癌细胞株A549、宫颈癌细胞株Hela和乳腺癌细胞株MCF-7的细胞毒活性。结果表明化合物2,3和7对所测试的人源肿瘤细胞具有较弱的抑制活性。
The chemical constituents of cicadae were separated by column chromatography with silica gel, MPLC and preparative HPLC. Their structures were identified by their physicochemical properties and MS and NMR spectral data. Nine compounds were isolated and identified from 95% ethanolic extracts of Cicadaceae, including 5 acernikol-4 "-O-β-D-glucopyranoside (1), (7R, 8S) -dihydrodehydrodiconiferyl alcohol 9- O-β-D-glucopyranoside (2), (7R, 8S) -dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside (3), (7R, 8S) -dihydrodehydrodiconiferyl alcohol 9’-O- (4), (7R, 8S) -5-methoxydihydrofuran drodiconiferyl alcohol 4-O-β-D-glucopyranoside (5); 3 sucrose esters 3,6’-O- diferuloylsucrose (6) The compounds 1 ~ 5, 8, and 9 were isolated from the genus Cicadaceae for the first time.MTT method was used to determine the activity of the compound The cytotoxic activity against lung cancer cell line A549, cervical cancer cell line Hela and breast cancer cell line MCF-7 was tested.The results showed that compounds 2, 3 and 7 had weaker inhibitory activity on the human tumor cells tested.