论文部分内容阅读
目的 探讨川芎嗪、黄芪对脑缺血再灌注后神经细胞凋亡及Fos蛋白表达的影响。方法 48只雄性SD大鼠,随机等分成4组,每组12只。A组:假手术组;B组:生理盐水对照组;C组:川芎嗪治疗组;D组:黄芪治疗组。采用TUNEL法及免疫组化法分别检测各组大鼠脑组织神经细胞凋亡及Fos蛋白的表达。结果 与A组比较,B组大鼠神经细胞凋亡数目及。Fos蛋白阳性细胞数目增多,Fos蛋白阳性细胞平均灰度值下降(P<0.01);与B组比较,C组、D组大鼠神经细胞凋亡数目及Fos蛋白阳性细胞数目下降,Fos蛋白阳性细胞平均灰度值升高(P<0.01)。结论 川芎嗪、黄芪均可能通过抑制脑缺血再灌注后Fos蛋白表达而减少神经细胞凋亡。
Objective To investigate the effects of tetramethylpyrazine and astragalus on neuronal apoptosis and Fos protein expression after cerebral ischemia-reperfusion. Methods Forty-eight male SD rats were randomly divided into 4 groups of 12 in each group. Group A: Sham group; Group B: saline control group; Group C: Ligustrazine treatment group; Group D: Huangqi treatment group. TUNEL assay and immunohistochemistry were used to detect the neuronal apoptosis and Fos protein expression in rat brain. Results Compared with group A, the number of apoptotic neurons in group B was significant. The number of Fos protein positive cells increased, and the mean gray value of Fos protein positive cells decreased (P<0.01). Compared with group B, the number of neuronal apoptosis and the number of Fos protein positive cells decreased in group C and D, Fos protein positive The average gray value of cells increased (P<0.01). Conclusion Ligustrazine and Astragalus membranaceus may reduce neuronal apoptosis by inhibiting Fos protein expression after cerebral ischemia-reperfusion.