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人骨髓瘤细胞系的建立,是进行人-人淋巴细胞杂交瘤技术必不可少的要素。国外现已建有几个人骨髓瘤细胞系统(如Crose的GM1500B、TG_(12),Kaplan的U_(226)AR和Clark的RPMI_(8226)等)都还存在些问题仍未完全解决。我们经过多次筛选培养,于1983年10月从1例非分泌型多发性骨髓瘤患者体中分离培养的一株骨
The establishment of human myeloma cell lines is an essential element in the technology of human-human lymphocyte hybridomas. There are still some problems that the foreign human myeloma cell systems (such as GM1500B, TG_ (12) in Crose, U_ (226) AR in Kaplan and RPMI_ (8226) in Clark) are still not completely solved. After several screening and culture, we isolated and cultured a bone from one non-secreting multiple myeloma patient in October 1983