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一、微电极发展概况一般把能记录单个细胞或单根神经纤维活动的电极称之为微电极。 1989年,英国生理学家Hodgkin把微滴管(micropipette)刺入枪乌(鱼侧)的巨轴突,正确测量了膜电位的绝对值,这可以说是微电极的首次应用。接着,Renshow首先将微电极用于中枢神经系统的研究。他用直径为10微米的不诱钢微电极成功地记录了脊髓前角抑制性中间神经元的电活动。 1949年,凌宁和Gerard把微滴管拉得更细,并最先将这种微电极穿刺入骨骼肌细胞,测定了蛙肌细胞的膜电位。之后,Eccles继续Sherrington学派的工作,并将微电极实验技术应
First, the general development of microelectrode Generally can record single cell or single nerve fiber activity of the electrode called the microelectrode. In 1989, Hodgkin, a British physiologist, stuck a micropipette into the giant axon of the guayule (fish side) and measured the absolute value of the membrane potential correctly. This is the first application of the microelectrode. Next, Renshow first used microelectrodes for CNS research. He successfully recorded the electrical activity of the inhibitory interneurons in the anterior horn of the spinal cord using a magnetically-induced microelectrode with a diameter of 10 microns. In 1949, Lingning and Gerard pulled the tube a little finer and first punctured the microelectrode into skeletal muscle cells and determined the membrane potential of frog muscle cells. Afterwards, Eccles continued the Sherrington School’s work and applied the microelectrode experimental technique