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(一)对表皮LDH、ATP酶、組織蛋白酶的某些性貭作了初步研究。(二)在甲基胆蒽誘发皮肤癌过程中,观察到:(1)組織提取液的蛋白貭含量有所增高;(2)GDH、GOT、谷氨酰胺酶的活性显著降低;(3)LDH的活性显著增高;(4)組織蛋白酶与二肽酶活性在增生期有所增高;在乳头瘤及皮肤癌則略有降低;(5)ATP酶活性无显著变化;(6)在增生期蛋白—SH基增多,非蛋白—SH基減少;乳头瘤与皮肤癌的蛋白—SH基接近正常,非蛋白—SH基增多。(三)在皮肤引癌早期,如滴甲基胆蒽一次后2斗小时,GDH活性即显著下降,而LDH活性仍接近正常。(四)滴非致癌物,蒽对表皮GDH及LDH活性无显著影响。(五)結合形态学的观察,对上述酶活性及—SH基变化在皮肤癌变中的可能意义,作了簡短討論。
(a) A preliminary study was conducted on certain characteristics of epidermal LDH, ATPase, and cathepsin. (b) In the process of skin cancer induced by methylcholanthrene, it was observed that: (1) the peptone content of the tissue extract increased; (2) the activity of GDH, GOT, and glutaminase decreased significantly; (3) ) LDH activity increased significantly; (4) Cathepsin and dipeptidase activity increased during proliferative phase; slightly decreased in papilloma and skin cancer; (5) no significant change in ATPase activity; (6) in hyperplasia Period protein-SH group increased, non-protein-SH group decreased; papilloma and skin cancer protein-SH group near normal, non-protein-SH group increased. (3) In the early stage of skin cancer induction, such as two hours after a single dose of methylguanidine, the activity of GDH decreased significantly, while the LDH activity remained close to normal. (D) Non-carcinogens were not found, and no significant effect of earthworms on epidermal GDH and LDH activity was observed. (e) In conjunction with morphological observations, a brief discussion was made of the possible significance of the above-mentioned enzyme activity and the change of SH-group in the skin carcinogenesis.