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目的分析溃疡性结肠炎(UC)患者末梢血的串联质谱氨基酸含量的变化,为早期治疗提供依据。方法选择21例UC患者为研究组,46例健康体检正常者为对照组,均采用串联质谱技术对末梢血中氨基酸进行检测,比较两组研究对象串联质谱检测结果。结果经分析研究组患者脯氨酸、色氨酸和组氨酸浓度均低于对照组,差异具有统计学意义(P<0.05)。对这3种氨基酸指标进行线性回归分析,并得到了以下的Logistic线性回归方程模型:In[P/(1-P)]=2.615-0.005×Pro-0.023×His。根据向后剔除法,最终得到了在两组数据中差异最显著的2个指标,分别为脯氨酸、组氨酸,且都表现为下降,表示这2种氨基酸与UC有明显的关联。结论 UC患者中脯氨酸、色氨酸、组氨酸与健康体检正常者存在明显差异,提示UC患者存在着氨基酸代谢的变化,为UC的早期诊断及指导治疗提供依据。
Objective To analyze the change of amino acid content in tandem mass spectrometry of peripheral blood in patients with ulcerative colitis (UC), and to provide basis for early treatment. Methods Twenty-one patients with UC were selected as research group and 46 healthy controls as control group. Amino acid in peripheral blood was detected by tandem mass spectrometry, and the results of tandem mass spectrometry were compared between the two groups. Results The concentrations of proline, tryptophan and histidine in the study group were lower than those in the control group, the difference was statistically significant (P <0.05). Linear regression analysis of these three amino acid indicators, and get the following Logistic regression equation model: In [P / (1-P)] = 2.615-0.005 × Pro-0.023 × His. According to the backward culling method, the two indexes with the most significant differences between the two groups were obtained, which were proline and histidine, respectively, and all showed a decrease, indicating that the two amino acids were significantly associated with UC. CONCLUSIONS: Proline, tryptophan and histidine in patients with UC are significantly different from those in healthy subjects, suggesting that there is a change in amino acid metabolism in UC patients, which may provide evidence for the early diagnosis and treatment of UC.