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目的:研究三七总皂苷(PNS)抑制宫颈癌Hela细胞增殖的可能机制。方法:MTT法检测不同浓度PNS作用于Hela细胞24、48和72hHela细胞增殖率;检测PNS作用于Hela细胞24h荧光素酶报告基因(LUC)的变化;蛋白质印迹法检测200μg/mL PNS作用于Hela细胞4E-BP1、S6K1、mTOR及其磷酸化水平变化;ELISA法检测PNS作用于Hela细胞24hVEGF的变化。结果:PNS可以明显抑制Hela细胞生长,随时间延长及PNS剂量增加抑制率明显增高;LUC相对光单位值(RLU值)明显低于对照组,其S6K1、4E-BP1、mTOR蛋白表达及其磷酸化水平均明显降低,200μg/mL PNS作用于Hela细胞24h后其VEGF表达明显降低,差异均有统计学意义,P值均<0.01。结论:PNS能抑制Hela细胞增殖,其机制可能是PNS阻断mTOR信号通路,降低mRNA翻译效率,抑制蛋白质翻译起始复合物形成,从而抑制Hela细胞增殖。
Objective: To investigate the possible mechanism of Panax notoginseng saponins (PNS) in inhibiting Hela cell proliferation. Methods: MTT assay was used to detect the proliferation of Hela cells treated with PNS for 24, 48 and 72 hours. The changes of luciferase reporter gene (LUC) in Hela cells were detected by PNS. Western blotting was used to detect the effect of PNS on Hela cells The changes of 4E-BP1, S6K1, mTOR and phosphorylation of HeLa cells were detected by ELISA. Results: PNS could significantly inhibit the growth of Hela cells. With the prolongation of time and the increase of PNS dose, the inhibitory rate of PNS was significantly increased. The RLU value of LUC was significantly lower than that of control group. The expression of S6K1, 4E-BP1 and mTOR The levels of VEGF were significantly decreased in HepG2 cells treated with 200μg / mL PNS for 24h, the difference was statistically significant (P <0.01). CONCLUSION: PNS can inhibit the proliferation of Hela cells. The mechanism may be that PNS blocks mTOR signaling pathway, reduces mRNA translation efficiency, inhibits the formation of protein translation initiation complex and thus inhibits Hela cell proliferation.